Curcumin encapsulation in yeast glucan particles promotes its anti-inflammatory potential in vitro
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
31323374
DOI
10.1016/j.ijpharm.2019.118532
PII: S0378-5173(19)30576-9
Knihovny.cz E-zdroje
- Klíčová slova
- Curcumin, Drug carrier, Inflammation, Monocytes, Pharmaceutical composite, β-Glucan microparticles,
- MeSH
- antiflogistika aplikace a dávkování chemie MeSH
- glukany aplikace a dávkování chemie MeSH
- interleukin-1beta metabolismus MeSH
- kurkumin aplikace a dávkování chemie MeSH
- lidé MeSH
- lipopolysacharidy MeSH
- makrofágy účinky léků metabolismus MeSH
- monocyty účinky léků metabolismus MeSH
- NF-kappa B metabolismus MeSH
- reaktivní formy kyslíku metabolismus MeSH
- Saccharomyces cerevisiae MeSH
- THP-1 buňky MeSH
- TNF-alfa metabolismus MeSH
- transkripční faktor AP-1 metabolismus MeSH
- viabilita buněk účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antiflogistika MeSH
- glukany MeSH
- IL1B protein, human MeSH Prohlížeč
- interleukin-1beta MeSH
- kurkumin MeSH
- lipopolysacharidy MeSH
- NF-kappa B MeSH
- reaktivní formy kyslíku MeSH
- TNF protein, human MeSH Prohlížeč
- TNF-alfa MeSH
- transkripční faktor AP-1 MeSH
Glucan particles (GPs) from Saccharomyces cerevisiae are hollow shells that are composed mainly of β-1,3-d-glucan, which has demonstrated immunomodulatory and anti-inflammatory potential both in vitro and in vivo. Curcumin is a natural hydrophobic phenolic compound, which possesses a significant anti-inflammatory effect and is used as supportive therapy in the treatment of many inflammatory diseases. The aim of this study is to evaluate the possible synergic effect and other benefits of the co-application of GPs and curcumin in the form of pharmaceutical composites. GP/curcumin composites were prepared using controlled evaporation of the organic solvent and their anti-oxidative effect and anti-inflammatory potential were tested on THP1‑XBlue™‑MD2‑CD14 human monocytes cell line. The anti-oxidative effect was measured on pyocyanin-stimulated cells in vitro and the NF-κB/AP-1 signaling pathway on lipopolysaccharide pre-treated monocytes was chosen for anti-inflammatory assays. The secretion of pro-inflammatory cytokines TNF-α and IL-1β was evaluated as well. Results mostly showed a pro-oxidative activity of empty GPs, however, pharmaceutical composites demonstrated an anti-oxidative effect. The activity of NF-κB/AP-1 was substantially decreased by the tested GP/curcumin composites, which also caused the attenuation of cytokines secretion. The obtained results indicate a beneficial effect of the incorporation of curcumin into GPs.
Citace poskytuje Crossref.org
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