The SEVA platform (https://seva-plasmids.com) was launched one decade ago, both as a database (DB) and as a physical repository of plasmid vectors for genetic analysis and engineering of Gram-negative bacteria with a structure and nomenclature that follows a strict, fixed architecture of functional DNA segments. While the current update keeps the basic features of earlier versions, the platform has been upgraded not only with many more ready-to-use plasmids but also with features that expand the range of target species, harmonize DNA assembly methods and enable new applications. In particular, SEVA 4.0 includes (i) a sub-collection of plasmids for easing the composition of multiple DNA segments with MoClo/Golden Gate technology, (ii) vectors for Gram-positive bacteria and yeast and [iii] off-the-shelf constructs with built-in functionalities. A growing collection of plasmids that capture part of the standard-but not its entirety-has been compiled also into the DB and repository as a separate corpus (SEVAsib) because of its value as a resource for constructing and deploying phenotypes of interest. Maintenance and curation of the DB were accompanied by dedicated diffusion and communication channels that make the SEVA platform a popular resource for genetic analyses, genome editing and bioengineering of a large number of microorganisms.

Centro de Biotecnología y Genómica de Plantas Universidad Politécnica de Madrid Pozuelo de Alarcón 28223 Spain

Department of Experimental Biology Faculty of Science Masaryk University Brno 62500 Czech Republic

School of Computing Newcastle University NE4 5TG UK

Scienseed SL 28020 Madrid Spain

Systems Biology Department Centro Nacional de Biotecnología 28049 Cantoblanco Madrid Spain

The Novo Nordisk Foundation Center for Biosustainability Technical University of Denmark 2800 Kongens Lyngby Denmark

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An Initial Genome Editing Toolset for Caldimonas thermodepolymerans, the First Model of Thermophilic Polyhydroxyalkanoates Producer