Silver nanoparticles (Ag NPs) with antibacterial activity can be prepared in different ways. In our case, we used ecological green synthesis with Agrimonia eupatoria L. The plant extract was used with Ag NPs for the first time to prepare termosensitive in situ gels (ISGs). Such gels are used to heal human or animal skin and mucous membranes, as they can change from a liquid to solid state after application. Ag NPs were characterized with various techniques (FTIR, TEM, size distribution, zeta potential) and their antibacterial activity was tested against Staphylococcus aureus and Escherichia coli. In accordance with the TEM data, we prepared monodispersed spherical Ag NPs with an average size of about 20 nm. Organic active compounds from Agrimonia eupatoria L. were found on their surfaces using FTIR spectroscopy. Surprisingly, only the in situ gel with Ag NPs showed antibacterial activity against Escherichia coli, while Ag NPs alone did not. Ag NPs prepared via green synthesis using plants with medicinal properties and incorporated into ISGs have great potential for wound healing due to the antibacterial activity of Ag NPs and the dermatological activity of organic substances from plants.

Advanced Materials Department Jozef Stefan Institute SI 1000 Ljubljana Slovenia

Department of Chemistry Biochemistry and Biophysics Institute of Pharmaceutical Chemistry University of Veterinary Medicine and Pharmacy in Košice 041 81 Kosice Slovakia

Department of Mechanochemistry Institute of Geotechnics Slovak Academy of Sciences 040 01 Kosice Slovakia

Department of Microbiology and Immunology University of Veterinary Medicine and Pharmacy in Košice 041 81 Kosice Slovakia

Department of Pharmaceutical Technology Faculty of Pharmacy Masaryk University 601 77 Brno Czech Republic

Department of Pharmaceutical Technology Pharmacognosy and Botany University of Veterinary Medicine and Pharmacy in Košice 041 81 Kosice Slovakia

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