Genomic analysis of Paenibacillus larvae isolates from the Czech Republic and the neighbouring regions of Slovakia
Language English Country England, Great Britain Media print-electronic
Document type Journal Article
PubMed
36913910
DOI
10.1016/j.rvsc.2023.03.007
PII: S0034-5288(23)00072-3
Knihovny.cz E-resources
- Keywords
- AFB spread, American foulbrood, Epidemiology, Genotyping, Honeybee,
- MeSH
- Genomics MeSH
- Genotype MeSH
- Larva genetics microbiology MeSH
- Humans MeSH
- Multilocus Sequence Typing veterinary MeSH
- Paenibacillus larvae * genetics MeSH
- Bees MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Slovakia epidemiology MeSH
- United States MeSH
Paenibacillus larvae is the causative agent of American foulbrood (AFB), a devastating disease of honeybee larvae. In the Czech Republic, two large infested regions were recognised. This study aimed to analyse P. larvae strains occurring in the Czech Republic in the years 2016-2017 and to characterise the genetic structure of their population with the use of Enterobacterial Repetitive Intergenic Consensus genotyping (ERIC), multilocus sequence typing (MLST) and whole genome sequence (WGS) analysis. The results were complemented by the analysis of isolates collected in the year 2018 in areas of Slovakia located near the Czechia-Slovakia border. ERIC genotyping revealed that 78.9% of tested isolates belonged to the ERIC II genotype and 21.1% to ERIC I genotype. MLST showed six sequence types with ST10 and ST11 being the most frequent among isolates. Within six isolates we found discrepancies in correlations between MLST and ERIC genotypes. The use of MLST and WGS analysis of isolates revealed that each of the large infested geographic regions had its own dominating P. larvae strains. We assume that these strains represented primary sources of infection in the affected areas. In addition, the sporadic presence of strains identified by core genome analysis as genetically related was unveiled in geographically distant regions suggesting possible human-mediated transmission of AFB.
Institute of Chemistry Slovak Academy of Sciences Dúbravská cesta 9 84538 Bratislava Slovakia
Ptácy s r o Valašská Bystřice 194 756 27 Valašská Bystřice Czech Republic
Veterinary Research Institute Hudcova 296 70 62100 Brno Czech Republic
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