ArdB proteins are known to inhibit the activity of the type I restriction-modification (RM-I) system, in particular EcoKI (IA family). The mechanism of ArdB's activity still remains unknown; the spectrum of targets inhibited has been poorly studied. In this work, it was shown that the presence of the ardB gene from the R64 plasmid could suppress the activity of EcoAI endonuclease (IB family) in Escherichia coli TG1 cells. Due to the absence of specificity of ArdB to a certain RM-I system (it inhibits both the IA- and IB-family), it can be assumed that the mechanism of the anti-restriction activity of this protein does not depend on the sequence DNA at the recognition site nor the structure of the restriction enzyme of the RM-I systems.

Autonomous non profit educational organization Phystech Lyceum named after P L Kapitsa Dolgoprudny Moscow oblast 141707 Russia

Institute of Microbiology Academy of Sciences of the Czech Republic Vídeňská 1083 Praha 4 14220 Czech Republic

Laboratory of Structural Biology and Bioinformatics Institute of Microbiology Academy of Sciences of the Czech Republic Zamek 136 Nove Hrady 37333 Czech Republic

Moscow Institute of Physics and Technology Dolgoprudny Moscow oblast 141707 Russia

Moscow State University of Food Production Moscow 125080 Russia

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Broadness and specificity: ArdB, ArdA, and Ocr against various restriction-modification systems