Anti-Restriction Activity of ArdB Protein against EcoAI Endonuclease
Language Russian Country Russia (Federation) Media print
Document type English Abstract, Journal Article
- Keywords
- ArdB, EcoAI, RM-I, antirestriction,
- MeSH
- DNA MeSH
- Escherichia coli * genetics metabolism MeSH
- Plasmids genetics MeSH
- Escherichia coli Proteins * genetics chemistry MeSH
- DNA Restriction Enzymes genetics MeSH
- Publication type
- English Abstract MeSH
- Journal Article MeSH
- Names of Substances
- ardB protein, E coli MeSH Browser
- DNA MeSH
- Escherichia coli Proteins * MeSH
- DNA Restriction Enzymes MeSH
ArdB proteins are known to inhibit the activity of the type I restriction-modification (RM-I) system, in particular EcoKI (IA family). The mechanism of ArdB's activity still remains unknown; the spectrum of targets inhibited has been poorly studied. In this work, it was shown that the presence of the ardB gene from the R64 plasmid could suppress the activity of EcoAI endonuclease (IB family) in Escherichia coli TG1 cells. Due to the absence of specificity of ArdB to a certain RM-I system (it inhibits both the IA- and IB-family), it can be assumed that the mechanism of the anti-restriction activity of this protein does not depend on the sequence DNA at the recognition site nor the structure of the restriction enzyme of the RM-I systems.
Moscow Institute of Physics and Technology Dolgoprudny Moscow oblast 141707 Russia
Moscow State University of Food Production Moscow 125080 Russia
References provided by Crossref.org
Broadness and specificity: ArdB, ArdA, and Ocr against various restriction-modification systems
