A new species of Marsupella sect. Ustulatae Müll. Frib. ex R.M. Schust. is described following an integrated morphological and molecular-phylogenetic study which examined the recently found dioicous plants growing epilithically on acidic substrates in several mountain ranges of Portugal between Peneda-Gerês in the north and Serra da Monchique in the extreme south. Employed molecular markers (plastid trnF-trnT region and nuclear ribosomal ITS) confirmed the distinctness of the lineage from other currently recognized species in the section, and furthermore, previously neglected diversity within M. sprucei (Limpr.) Bernet was signaled. Although not yet confirmed outside Portugal, the newly reported species is probably not rare in the region and has likely been overlooked as M. funckii (F. Weber & D. Mohr) Dumort. or M. profunda Lindb. in the past.

Cerca dos Pomares CxP 409M 8670 052 Aljezur Portugal

Faculty of Science University of South Bohemia Branišovská 1760 370 05 České Budějovice Czech Republic

Royal Botanic Garden Edinburgh 20a Inverleith Row Edinburgh EH3 5LR UK

Zobrazit více v PubMed

Söderström L., Hagborg A., von Konrat M., Bartholomew-Began S., Bell D., Briscoe L., Brown E., Cargill D.C., da Costa D.P., Crandall-Stotler B.J., et al. World checklist of hornworts and liverworts. PhytoKeys. 2016;59:1–828. doi: 10.3897/phytokeys.59.6261. PubMed DOI PMC

Mamontov Y.S., Vilnet A.A., Konstantinova N.A., Bakalin V.A. Two new species of Gymnomitriaceae (Marchantiophyta) in the North Pacific. Bot. Pac. 2019;8:67–80. doi: 10.17581/bp.2019.08113. DOI

Bakalin V.A., Fedosov V.E., Fedorova A.V., Nguyen V.S. Integrative Taxonomic Revision of Marsupella (Gymnomitriaceae, Hepaticae) Reveals Neglected Diversity in Pacific Asia. Cryptogam. Bryol. 2019;40:59–85. doi: 10.5252/cryptogamie-bryologie2019v40a7. DOI

Bakalin V.A., Vilnet A.A., Mamontov Y.S., Schäfer-Verwimp A., Maltseva Y.D., Klimova K.G., Nguyen V.S., Choi S.S. Stolonicaulon: A Section-Puzzle within Marsupella (Gymnomitriaceae, Marchantiophyta) Plants. 2022;11:1596. doi: 10.3390/plants11121596. PubMed DOI PMC

Hodgetts N.G., Söderström L., Blockeel T.L., Caspari S., Ignatov M.S., Konstantinova N.A., Lockhart N., Papp B., Schröck C., Sim-Sim M., et al. An annotated checklist of bryophytes of Europe, Macaronesia and Cyprus. J. Bryol. 2020;42:1–116. doi: 10.1080/03736687.2019.1694329. DOI

Sérgio C., Garcia C., Sim-Sim M., Vieira C., Hespanhol H., Stow S. Atlas e Livro Vermelho dos Briófitos Ameaçados de Portugal. MUHNAC, Documenta; Lisboa, Spain: 2013.

Vilnet A.A., Konstantinova N.A., Troitsky A.V. Molecular insight on phylogeny and systematics of the Lophoziaceae, Scapaniaceae, Gymnomitriaceae and Jungermanniaceae. Arctoa. 2010;19:31–50. doi: 10.15298/arctoa.19.02. DOI

Porley R., Papp B., Söderström L., Hallingbäck T. European Bryophyte Conservation in the New Millennium. University of Malaya; Kuala Lumpur, Malaysia: 2008.

Paton J.A. The Liverwort Flora of the British Isles. Harley Books; Colchester, UK: 1999.

Bell D., Long D., Hollingsworth P. The Use of DNA Barcoding to Address Major Taxonomic Problems for Rare British Bryophytes. Royal Botanic Garden Edinburgh; Edinburgh, UK: 2013. Final Revised Report.

Dayrat B. Towards integrative taxonomy. Biol. J. Linn. Soc. 2005;85:407–415. doi: 10.1111/j.1095-8312.2005.00503.x. DOI

Ignatov M.S., Porley R.D., Ochyra R., Kuznetsova O.I., Ignatova E.A. Coscinodon monchiquensis R.D. Porley, Ochyra & Ignatova (Grimmiaceae), a new species from the Algarve, southern Portugal. J. Bryol. 2018;40:125–136. doi: 10.1080/03736687.2018.1427177. DOI

Porley R.D., Fedosov V., Plášek V., Fedorova A. Undiscovered Biodiversity of the European Moss Flora: Neodicranella hamulosa (Aongstroemiaceae), a New Genus and Species from SW Portugal. Plants. 2021;10:2289. doi: 10.3390/plants10112289. PubMed DOI PMC

Garcia C., Sérgio C., Jansen J. The bryophyte flora of the natural park of Serra da Estrela (Portugal): Conservation and Biogeographical approaches. Cryptogam. Bryol. 2008;29:49–73.

Sérgio C., Garcia C., Hespanhol H., Vieira C., Stow S., Long D. Diversidad de briófitos en el Parque Nacional Peneda-Gerês (Portugal): Seleccionando áreas importantes de plantas “Important Plant Areas (IPA)” basado en nuevos datos y anteriores referencias. Bot. Complut. 2012;36:39–50. doi: 10.5209/rev_BOCM.2012.v36.39443. DOI

Sérgio C., Schumacker R. Contribuiçao para o estudo da flora briológica do Parque Nacional da Peneda-Gerês. Port. Acta Biol. 1992;16:107–137.

Hespanhol H., Vieira C., Lomba Â., Séneca A. New data on bryophyte distribution in the Peneda-Gerês National Park (NW Portugal): The use of GIS for conservation remarks. Bol. Soc. Esp. Briol. 2005;26–27:59–74.

Sérgio C., Silva I. The bryophyte flora of the Vouga region (Aveiro, Beira Litoral) Port. Acta Biol. 2007;22:159–188.

Simmons M.P., Ochoterena H. Gaps as Characters in Sequence-Based Phylogenetic Analyses. Syst. Biol. 2000;49:369–381. doi: 10.1093/sysbio/49.2.369. PubMed DOI

Grolle R. Miscellanea Hepaticologica 151–160. Lindbergia. 1975;3:47–56.

Váňa J., Söderström L., Hagborg A., Von Konrat M., Engel J.J. Early Land Plants Today: Taxonomy, systematics and nomenclature of Gymnomitriaceae. Phytotaxa. 2010;11:1. doi: 10.11646/phytotaxa.11.1.1. DOI

Müller K. Die Lebermoose Deutschlands, Oesterreichs und der Schweiz. 2nd ed. Volume 6 E. Kummer; Leipzig, Germany: 1906–1911. Dr. L. Rabenhorst’s Kryptogamen-Flora von Deutschland, Oesterreich und der Schweiz.

Müller K. Die Lebermoose Europas (Musci hepatici) 3rd ed. Volume 6 Geest & Portig; Leipzig, Germany: 1956. Dr. L. Rabenhorst’s Kryptogamen-Flora von Deutschland, Oesterreich und der Schweiz.

Schiffner V.F. Kritische Bemerkungen über die europäische Lebermoose mit Bezug auf die Exemplare des Exsiccatenwerkes: Hepaticae europaeae exsiccatae. I. Serie. Sitzungsber. Deutsch. Naturwiss. Med. Ver. Böhmen “Lotos” Prag. 1901;49:75–130.

Limpricht K.G. Über neue Arten und Formen der Gattung Sarcoscyphus Corda. Jahresber. Schles. Ges. Vaterländische Cult. 1880;58:179–184.

Paton J.A. Marsupella profunda Lindb in Cornwall, new to the British Isles. J. Bryol. 1990;16:1–4. doi: 10.1179/jbr.1990.16.1.1. DOI

Konstantinova N.A. Marsupella. In FNA Editors, Gymnomitriaceae, Bryophyte Flora of North America; Provisional Publication, 2020. [(accessed on 18 March 2023)]. Available online: http://www.mobot.org/plantscience/bfna/V3/Gymnomitriaceae.htm.

Schiffner V. Studien über kritische Arten der Gattungen Gymnomitrium und Marsupella. (Fortsetzung.) Oesterr. Bot. Z. 1903;53:166–172. doi: 10.1007/BF01678264. DOI

Damsholt K. Illustrated Flora of Nordic Liverworts and Hornworts. Nordic Bryological Society; Lund, Sweden: 2002.

Damsholt K. The Liverworts of Greenland. Nordic Bryological Society; Lund, Sweden: 2013.

Váňa J. Notes on the genus Marsupella s. lat. (Gymnomitriaceae, Hepaticae) 1–10. Infrageneric taxa. Bryobrothera. 1999;5:221–229.

Grolle R. Verzeichnis der Lebermoose Europas und benachbarter Gebiete. Feddes Repert. 1976;76:171–279. doi: 10.1002/fedr.19760870303. DOI

Duda J., Váňa J. Rozšíření játrovek v Československu—XXXVII [Die Verbreitung der Lebermoose in der Tschechoslowakei—XXXVII] Časopis Slez. Muz., Sér. A, Vědy Přír. 1983;32:97–110.

Malato Beliz J. A Serra de Monchique: FLORA e Vegetação. Serviço Nacional de Parques Reservas e Património Paisagístico; Lisboa, Portugal: 1982. Parques Naturais.

González-Clavijo E.J., Valadares V. A Estrutura do Complexo de Monchique; Proceedings of the Actas do VI Congresso Nacional de Geologia Ciências da Terra (UNL); Lisboa, Portugal. 4–6 June 2003; pp. 37–40.

Rock N.M.S. Petrology and Petrogenesis of the Monchique Alkaline Complex, Southern Portugal. J. Petrol. 1978;19:171–214. doi: 10.1093/petrology/19.2.171. DOI

Bernard-Griffiths J., Gruau G., Cornen G., Azambre B., Mace J. Continental Lithospheric Contribution to Alkaline Magmatism: Isotopic (Nd, Sr, Pb) and Geochemical (REE) Evidence from Serra de Monchique and Mount Ormonde Complexes. J. Petrol. 1997;38:115–132. doi: 10.1093/petroj/38.1.115. DOI

Lara F., Porley R.D., Draper I., Aleffi M., Garcia C., Garilleti R. Ulota s.l. (Orthotrichaceae, Bryidae) at southernmost Mediterranean localities: Not a simple matter. Pl. Biosyst. 2022;156:1448–1455. doi: 10.1080/11263504.2022.2056652. DOI

Hill M.O., Preston C.D. The geographical relationships of British and Irish bryophytes. J. Bryol. 1998;20:127–226. doi: 10.1179/jbr.1998.20.1.127. DOI

Patiño J., Bisang I., Goffinet B., Hedenäs L., McDaniel S., Pressel S., Stech M., Ah-Peng C., Bergamini A., Caners R.T., et al. Unveiling the nature of a miniature world: A horizon scan of fundamental questions in bryology. J. Bryol. 2022;44:1–34. doi: 10.1080/03736687.2022.2054615. DOI

Pörtner D.C., Roberts M., Tignor E.S., Poloczanska K., Mintenbeck A., Alegría M., Craig S., Langsdorf S., Löschke V., Möller A., et al. IPCC Climate Change 2022: Impacts, Adaptation, and Vulnerability. Contribution of Working Group II to the Sixth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press; Cambridge, UK: New York, NY, USA: 2022.

Mitchell R.J., Simonson W., Flegg L.A., Santos P., Hall J. A comparison of the resilience of four habitats to fire, and the implications of changes in community composition for conservation: A case study from the Serra de Monchique, Portugal. Plant Ecol. Divers. 2009;2:45–56. doi: 10.1080/17550870902752421. DOI

Tedim F., Leone V., Amraoui M., Bouillon C., Coughlan M., Delogu G., Fernandes P., Ferreira C., McCaffrey S., McGee T., et al. Defining Extreme Wildfire Events: Difficulties, Challenges, and Impacts. Fire. 2018;1:9. doi: 10.3390/fire1010009. DOI

Sérgio C., Bergamini A., Garcia C., Garilleti R., Infante M., Porley R.D. Marsupella profunda. The IUCN Red List of Threatened Species 2019: E.T39195A87726839 2019. [(accessed on 18 March 2023)]. Available online: https://www.gbif.org/fr/species/176752393. DOI

Kučera J., Kuznetsova O.I., Manukjanová A., Ignatov M.S. A phylogenetic revision of the genus Hypnum: Towards completion. Taxon. 2019;68:628–660. doi: 10.1002/tax.12095. DOI

Taberlet P., Gielly L., Pautou G., Bouvet J. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant. Mol. Biol. 1991;17:1105–1109. doi: 10.1007/BF00037152. PubMed DOI

Katoh K., Rozewicki J., Yamada K.D. MAFFT online service: Multiple sequence alignment, interactive sequence choice and visualization. Brief. Bioinform. 2019;20:1160–1166. doi: 10.1093/bib/bbx108. PubMed DOI PMC

Ronquist F., Teslenko M., van der Mark P., Ayres D.L., Darling A., Höhna S., Larget B., Liu L., Suchard M.A., Huelsenbeck J.P. MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space. Syst. Biol. 2012;61:539–542. doi: 10.1093/sysbio/sys029. PubMed DOI PMC

Stamatakis A. RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics. 2014;30:1312–1313. doi: 10.1093/bioinformatics/btu033. PubMed DOI PMC

Stöver B.C., Müller K.F. TreeGraph 2: Combining and visualizing evidence from different phylogenetic analyses. BMC Bioinform. 2010;11:7. doi: 10.1186/1471-2105-11-7. PubMed DOI PMC

Schuster R.M. The Hepaticae and Anthocerotae of North America. Volume III Columbia University Press; New York, NY, USA: London, UK: 1974.