The genus Aciculosporium (Clavicipitaceae, Hypocreales, Ascomycota)was established in 1908 for A. take , which is the causal fungus of witches' broom of bamboo. Although the original description was valid at that time, a type specimen for A. take has not been designated. To standardize the use of this genus and species name, a neotypification and reference specimen of A. take are proposed. Multilocus phylogenetic analyses based on DNA sequences from 28S rDNA, TEF, Tub2, Mcm7, and RPB2 revealed that A.sasicola is from a different lineage to A. take, and other specimens from wavyleaf basket grass (Oplismenus undulatifolius) represent a distinct species proposed here as Aciculosporium oplismeni sp. nov. Chemical analysis using mass spectrometry and nuclear magnetic resonance spectroscopy showed that A. take produces four proline-containing cyclic dipeptides, which are moieties of ergot alkaloids. However, ergot alkaloids, lolines, peramine, indole-diterpenes, and lolitrem were not detected in the culture solvent. This study offers clarification of the lineage and morphology of this genus.

Department of Organic Chemistry Hoshi University

Ishikawa Prefectural University

Laboratory of Fungal Genetics and Metabolism Institute of Microbiology of the Czech Academy of Sciences

Mountain Science Center Sugadaira Research Station University of Tsukuba

Zobrazit více v PubMed

Carrieri, R., Borriello, G., Piccirillo, G., Lahoz, E., Sorrentino, R., Cermola, M., Censi, S., Grauso, L., Mangoni, A., & Vinale, F. (. 2020). Antibiotic Activity of a Paraphaeosphaeria sporulosa-Produced Diketopiperazine against Salmonella enterica. Journal of Fungi, 6, 1- 9. https://doi.org/10.3390/jof6020083. PubMed PMC

Chanclud, E. & Morel, J. (2016). Plant hormones: a fungal point of view. Molecular Plant Pathology, 17, 1289-1297. https://doi.org/10.1111/mpp.12393. PubMed PMC

Ehmann, A. (1977). The van URK-Salkowski reagent — a sensitive and specific chromogenic reagent for silica gel thin-layer chromatographic detection and identification of indole derivatives. Journal of Chromatography A, 132, 267-276. https://doi.org/10.1016/S0021-9673(00)89300-0 PubMed

Fdhila, F., Vázquez, V., Sánchez, J. L., Riguera, R., (2003). DD-Diketopiperazines: Antibiotics active against Vibrio anguillarum isolated from marine bacteria associated with cultures of Pecten maximus. Journal of Natural Products, 66, 1299-1301. https://doi.org/10.1021/np030233e. PubMed

Hara, K. (1908). On witches' broom disease of bamboo(in Japnanese). Gifuken Nokai Zasshi 20, 13-14.

Hara, K. (1918), Takeno Tengusubyo(in Japnanese). Dainihon Sanrin Kaiho, 422, 40-43.

Hino, I. (1962) Pathogenic fungus of the witches' broom disease of bamboos (In Japanese). Transactions of the Mycological Society of Japan, 3, 111-113.

Izumitsu, K., Hatoh, K., Sumita, T., Kitade, Y., Morita, A., Gafur, A., Ohta, A., Kawai, M., Yamanaka, T., Neda, H., Ota, Y.,& Tanaka, C. (2012). Rapid and simple preparation of mushroom DNA directly from colonies and fruiting bodies for PCR. Mycoscience, 53, 396- 401. https://doi.org/10.1007/ S10267-012-0182-3.

Kobayashi, M. (2017). The illustrated book of plant systematics in color. Bambusoideae in Japan. Hokuryukan, Tokyo, pp 435.

Kwon, O. S., Park, S. H., Yun, B. S., Pyun, Y. R., & Kim, C. J. (2001). Cyclo(D-Pro-L-Val ), a specific β-glucosidase inhibitor produced by Aspergillus sp. F70609. The Journal of Antibiotics, 54, 179-181 https://doi.org/10.7164/antibiotics.54.179 PubMed

Magnusson, J., Ström, K., Roos, S., Sjögren, J., Schnürer, J. (2003). Broad and complex antifungal activity among environmental isolates of lactic acid bacteria. FEMS Microbiology Letters, 219, 129-135 https://doi.org/10.1016/S0378-1097(02)01207-7. PubMed

Miyake, I., (1908). On witches' broom disease of bamboo (In Japanese). Botanical Magazine Tokyo, 22, 305-307. https://doi.org/10.15281/jplantres1887.22.259_305

Oguchi, T., (2001). Aciculosporium sasicola sp. nov. on witches' broom of Sasa senanensis. Mycoscience, 42, 217-221. https://doi.org/10.1007/BF02464140

Pažoutová, S., Kolařík, M. & Kolínská, R., (2004). Pleomorphic conidiation in Claviceps. Mycological Research 108, 126-135. https://doi.org/10.1017/S0953756203009067 PubMed

Píchová, K., Pažoutová, S., Kostovčík, M., Chudíčková, M., Stodůlková, E., Novák, P., Flieger, M., van der Linde, E., & Kolařík, M., (2018). Evolutionary history of ergot with a new infrageneric classification (Hypocreales: Clavicipitaceae: Claviceps). Molecular Phylogenetics and Evolution, 123, 73-87. https://doi.org/10.1016/j.ympev.2018.02.013 PubMed

Schardl, C. L., Young, C. A., Hesse, U., Amyotte, S. G., Andreeva, K., Calie, P. J., Zeng, Z., (2013). Plant-symbiotic fungi as chemical engineers: multi-genome analysis of the Clavicipitaceae reveals dynamics of alkaloid loci. PLoS Genetics 9, e1003323. https://doi.org/10.1371/journal.pgen.1003323 PubMed PMC

Schardl, C. L., Young, C. A., Moore, N., Krom, N., Dupont, P. Y., Pan, J., Florea, S., Webb, J. S., Jaromczyk, J., Jaromczyk, J. W., Cox, M. P., & Farman, M. L., (2014). Genomes of plant-associated Clavicipitaceae. Advances in Botanical Research, 70, 291-327.https://doi.org/10.1016/B978-0-12-397940-7.00010-0 .

Song, Q.-Y., Nan, Z.-B., Gao, K., Song, H., Tian, P., Zhang, X.-X., Li, C.-J., Xu, W.-B., & Li, X.-Z. (2015). Antifungal, phytotoxic, and cytotoxic activities of metabolites from Epichloë bromicola, a fungus obtained from Elymus tangutorum grass. Journal of Agricultural and Food Chemistry, 63, 8787-8792.https://doi.org/10.1021/acs.jafc.5b04260. PubMed

Sullivan, R., Bergen, M. S., Patel, R., Bills, G. F., Alderman, S. C., Spatafora, J. W., & White, J. F. (2001).Features and phylogenetic status of an enigmatic clavicipitalean fungus Neoclaviceps monostipa gen. et sp. nov. Mycologia, 93, 90-99. https://doi.org/10.2307/3761608.

Swofford, D L. (2003). PAUP 4.0b10: Phylogenetic analysis using parsimony. Sunderland: , MA: Sinauer Associates.

Takaya, Y., Furukawa, T., Miura, S., Akutagawa, T., Hotta, Y., Ishikawa, N., &Niwa, M. (2007).Antioxidant Constituents in Distillation Residue of Awamori Spirits. Agricultural and Food Chemistry, 55, 75-79. https://doi.org/10.1021/jf062029d. PubMed

Tanaka, E. (2009). Specific in situ visualization of the pathogenic endophytic fungus Aciculosporium take, the cause of witches’ broom in bamboo. . Applied and Environmental Microbiology, 75. 4829-4834https://doi.org/10.1128/AEM.00635-09. PubMed PMC

Tanaka, E. (2010). Mechanisms of bamboo witches’ broom symptom development caused by endophytic/epiphytic fungi. Plant Signaling & Behavior 5, 415-418. PubMed PMC

Tanaka, E., Shrestha, B., Shivas, R. G. (2020). Commelinaceomyces gen. nov. for four clavicipitaceous species misplaced in Ustilago that infect Commelinaceae. Mycologia 112, 3,649-660. https://doi.org/10.1080/00275514.2020.1745524. PubMed

Tanaka, E., Tanaka, C., Ishihara, A., Kuwahara, Y., & Tsuda, M., (2003). Indole-3-acetic acid biosynthesis in Aciculosporium take, a causal agent of witches' broom of bamboo. Journal of General Plant Pathology, 69, 1-6. https://doi.org/10.1007/s10327-002-0002-2.

Tanaka, E., Tanaka, C., &Tsuda, M. (2002). Transmission and intraspecific variation of Aciculosporium take, the causal agent of witches' broom of bamboo (In Japanese). Forest Research, Kyoto, 74, 13-20.

Tsuda, M., Shimizu, K., Matsumura, K., Tanaka, E., Tanaka, C., & Doi, Y. (1997). Host range of Aciculosporium take, the causal agent of witches' broom of bamboo plants. Bulletin of the National Science Museum. Series B, Botany, 23, 25-34.

Walker, J. (2004). Claviceps phalaridis in Australia: Biology, pathology and taxonomy with a description of the new genus Cepsiclava (Hypocreales, Clavicipitaceae). Australasian Plant Pathology, 33, 211-239. https://doi.org/10.1071/AP04001.

Wang, X., Li, Y., Zhang, X., Lai, D., Zhou, L. (2017). Structural diversity and biological activities of the cyclodipeptides from fungi Molecules, 22, 2026,https://doi.org/10.3390/molecules22122026. PubMed PMC

Wang, Y., Mueller, U. G., &Clardy, J. O. N.(1999). Antifungal diketopiperazines from symbiotic fungus of fungus-growing ant Cyphomyrmex minutus. Journal of Chemical Ecology, 25. 935-941. https://doi.org/10.1023/A:1020861221126.

Yan, P., Song, Y., Sakuno, E., Nakajima, H., Nakagawa, H., & Yabe, K. (2004). Cyclo ( L-Leucyl-L-Prolyl ) produced by Achromobacter xylosoxidans inhibits aflatoxin production by Aspergillus parasiticus. Applied and Environmental Microbiology, 70, 7466-7473. https://doi.org/10.1128/AEM.70.12.7466-7473.2004 PubMed PMC

Zhou, L. Y., Zhang, X. X., Li, C. J., Christensen, M. J., & Nan, Z. B., (2015). Antifungal activity and phytochemical investigation of the asexual endophyte of Epichloë sp. from Festuca sinensis. Science China Life Sciences, 58, 821-826. https://doi.org/10.1007/s11427-015-4845-0. PubMed

In search of lost ergots: phylogenetic re-evaluation of Claviceps species in Japan and their biogeographic patterns revealed