Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica
Language English Country Germany Media print-electronic
Document type Journal Article
PubMed
37167755
DOI
10.1016/j.syapm.2023.126424
PII: S0723-2020(23)00033-4
Knihovny.cz E-resources
- Keywords
- Antarctica, P. petrae sp. nov., Pseudomonas, Regolith, Species description, Taxonomy,
- MeSH
- Genes, Bacterial * MeSH
- DNA, Bacterial genetics MeSH
- DNA MeSH
- Phospholipids * analysis MeSH
- Phylogeny MeSH
- Fatty Acids analysis MeSH
- Pseudomonas MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Bacterial Typing Techniques MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Antarctic Regions MeSH
- Names of Substances
- DNA, Bacterial MeSH
- DNA MeSH
- Phospholipids * MeSH
- Fatty Acids MeSH
- RNA, Ribosomal, 16S MeSH
A polyphasic taxonomic approach was used to characterize the four strains P2653T, P2652, P2498, and P2647, isolated from Antarctic regolith samples. Initial genotype screening performed by PCR fingerprinting based on repetitive sequences showed that the isolates studied formed a coherent cluster separated from the other Pseudomonas species. Identification results based on 16S rRNA gene sequences showed the highest sequence similarity with Pseudomonas graminis (99.7%), which was confirmed by multilocus sequence analysis using the rpoB, rpoD, and gyrB genes. Genome sequence comparison of P2653T with the most related P. graminis type strain DSM 11363T revealed an average nucleotide identity of 92.1% and a digital DNA-DNA hybridization value of 46.6%. The major fatty acids for all Antarctic strains were C16:0, Summed Feature 3 (C16:1ω7c/C16:1ω6c) and Summed Feature 8 (C18:1ω7c/C18:1ω6c). The predominant respiratory quinone was Q-9, and the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol. The regolith strains could be differentiated from related species by the absence of arginine dihydrolase, ornithine and lysine decarboxylase and by negative tyrosine hydrolysis. The results of this polyphasic study allowed the genotypic and phenotypic differentiation of four analysed strains from the closest related species, which confirmed that the strains represent a novel species within the genus Pseudomonas, for which the name Pseudomonas petrae sp. nov. is proposed with P2653T (CCM 8850T = DSM 112068T = LMG 30619T) as the type strain.
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