Kingella kingae RtxA toxin interacts with sialylated gangliosides
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
37315629
DOI
10.1016/j.micpath.2023.106200
PII: S0882-4010(23)00233-4
Knihovny.cz E-zdroje
- Klíčová slova
- Gangliosides, Kingella kingae, RTX toxins, RtxA, Sialic acid, Vesicles,
- MeSH
- bakteriální toxiny * metabolismus MeSH
- buněčná membrána metabolismus MeSH
- cytotoxiny metabolismus MeSH
- dítě MeSH
- faktory virulence metabolismus MeSH
- Kingella kingae * metabolismus MeSH
- lidé MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- bakteriální toxiny * MeSH
- cytotoxiny MeSH
- faktory virulence MeSH
The membrane-damaging RTX family cytotoxin RtxA is a key virulence factor of the emerging pediatric pathogen Kingella kingae, but little is known about the mechanism of RtxA binding to host cells. While we have previously shown that RtxA binds cell surface glycoproteins, here we demonstrate that the toxin also binds different types of gangliosides. The recognition of gangliosides by RtxA depended on sialic acid side groups of ganglioside glycans. Moreover, binding of RtxA to epithelial cells was significantly decreased in the presence of free sialylated gangliosides, which inhibited cytotoxic activity of the toxin. These results suggest that RtxA utilizes sialylated gangliosides as ubiquitous cell membrane receptor molecules on host cells to exert its cytotoxic action and support K. kingae infection.
Citace poskytuje Crossref.org
Kingella kingae cytotoxin RtxA induces an innate immune response in oral epithelial cells
