The novelty of this study is the investigation of the effect of different heat treatments and, simultaneously, the effect of different fat levels on the quality of pork liver pâtés. Hence, this study aimed to evaluate the effect of heat treatment and fat content on selected properties of pork liver pâté. For this purpose, four batches of pâtés with two different fat contents (30 and 40% (w/w)) and two different heat treatments (pasteurisation: target temperature 70 °C, holding time of 10 min; sterilisation: target temperature 122 °C, holding time of 10 min) were manufactured. Chemical (pH, dry matter, crude protein, total lipid, ammonia, and thiobarbituric acid reactive substances (TBARS)), microbiological, colour, textural, rheological, and sensory analyses were performed. Both different heat treatment and fat content affected most of the parameters observed. Sterilisation ensured the commercial sterility of the manufactured pâtés, resulting in elevated TBARS values, hardness, cohesiveness, gumminess and springiness, and increased rheological parameters (G', G″, G*, and η*), as well as colour changes (decrease in L* and increase in a*, b*, and C* values) and deterioration of appearance, consistency, and flavour also being detected (p < 0.05). Higher fat content caused similar variations in the textural and viscoelastic properties, i.e., the increase in hardness, cohesiveness, gumminess and springiness, and also in G', G″, G*, and η* (p < 0.05). However, the colour and sensorial parameters changed in different ways compared to the changes induced by the sterilisation effect. Overall, the observed changes might not be desirable for some consumers and further research would be appropriate to improve especially the sensorial attributes of sterilised pork liver pâtés.

Department of Food Technology Faculty of Technology Tomas Bata University in Zlin 760 01 Zlin Czech Republic

Laboratory of Food Quality and Safety Research Department of Logistics Faculty of Military Leadership University of Defence 662 10 Brno Czech Republic

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Lorenzo J.M., Pateiro M., García Fontán M.C., Carballo J. Effect of fat content on physical, microbial, lipid and protein changes during chill storage of foal liver pâté. Food Chem. 2014;155:57–63. doi: 10.1016/j.foodchem.2014.01.038. PubMed DOI

Amaral D.S., Silva F.A.P., Bezerra T.K.A., Arcanjo N.M.O., Guerra I.C.D., Dalmás P.S., Madruga M.S. Effect of storage time and packaging on the quality of lamb pâté prepared with variety meat. Food Packag. Shelf Life. 2015;3:39–46. doi: 10.1016/j.fpsl.2014.10.004. DOI

Momchilova M.M., Petrova T.V., Gradinarska-Ivanova D.N., Yordanov D.G. Emulsion and inulin stability of meat pate with reduced fat content as a function of sterilization regimes. Food Sci. Technol. 2021;41:980–986. doi: 10.1590/fst.27420. DOI

Martins A.J., Lorenzo J.M., Franco D., Pateiro M., Domínguez R., Munekata P.E.S., Pastrana L.M., Vicente A.A., Cunha R.L., Cerqueira M.A. Characterization of enriched meat-based pâté manufactured with oleogels as fat substitues. Gels. 2020;6:17. doi: 10.3390/gels6020017. PubMed DOI PMC

Domínguez R., Pateiro M., Munekata P.E.S., Campagnol P.C.B., Lorenzo J.M. Influence of partial pork backfat replacement by fish oil on nutritional and technological properties of liver pâté. Eur. J. Lipid Sci. Technol. 2017;119:1600178. doi: 10.1002/ejlt.201600178. DOI

Hamzeh A., Azizieh A., Yazagy S. The effect of the fat percentage and liver type in the stability and pH value of localy prepared liver pate. Int. Food Res. J. 2016;23:1131–1135.

Ugalde-Benítez V. Meat emulsions. In: Hui Y.H., editor. Handbook of Meat and Meat Processing. 2nd ed. CRC Press; Boca Raton, FL, USA: 2012. pp. 447–456.

Rezler R., Krzywdzińska-Bartkowiak M., Piątek M. The influence of the substitution of fat with modified starch on the quality of pork liver pâtés. LWT. 2021;135:110264. doi: 10.1016/j.lwt.2020.110264. DOI

Lorenzo J.M., Pateiro M. Influence of fat content on physico-chemical and oxidative stability of foal liver pâté. Meat Sci. 2013;95:330–335. doi: 10.1016/j.meatsci.2013.04.045. PubMed DOI

Estévez M., Ventanas S., Cava R. Effect of natural and synthetic antioxidants on protein oxidation and colour and texture changes in refrigerated stored porcine liver pâté. Meat Sci. 2006;74:396–403. doi: 10.1016/j.meatsci.2006.04.010. PubMed DOI

Doolaege E.H.A., Vossen E., Raes K., De Meulenaer B., Verhé R., Paelinck H., De Smet S. Effect of rosemary extract dose on lipid oxidation, colour stability, and antioxidant concentrations, in reduced nitrite liver pâtés. Meat Sci. 2012;90:925–931. doi: 10.1016/j.meatsci.2011.11.034. PubMed DOI

Polak T., Žlender B., Lušnic M., Gašperlin L. Effects of coenzyme Q10, α-tocopherol and ascorbic acid on oxidation of cholesterol in chicken liver pâté. LWT. 2011;44:1052–1058. doi: 10.1016/j.lwt.2010.10.010. DOI

Guerrero-Legarreta I., García-Barrientos R. Thermal technology. In: Hui Y.H., editor. Handbook of Meat and Meat Processing. 2nd ed. CRC Press; Boca Raton, FL, USA: 2012. pp. 523–530.

Deak T., Farkas J. Microbiology of Thermally Preserved Foods. Canning and Novel Physical Methods. DEStech Publications; Lancaster, PA, USA: 2013.

Li H., Wu Y., Hou D., Zhao S., Li D., Wang X., Li H., Yu J. Effects of pre-emulsification with whey protein and high temperature sterilisation on texture, functional characteristics and Maillard reaction products of room temperature stored processed cheese. Int. Dairy. J. 2023;140:105610. doi: 10.1016/j.idairyj.2023.105610. DOI

Bubelová Z., Černíková M., Buňková L., Talár J., Zajíček V., Foltin P., Buňka F. Quality changes of long-life foods during three-month storage at different temperatures. Potravin. Slovak. J. Food Sci. 2017;11:43–51. doi: 10.5219/688. PubMed DOI

Šopík T., Lazárková Z., Salek R.N., Talár J., Purevdorj K., Buňková L., Foltin P., Jančová P., Novotný M., Gál R., et al. Changes in the quality attributes of selected long-life food at four different temperatures over prolonged storage. Foods. 2022;11:2004. doi: 10.3390/foods11142004. PubMed DOI PMC

Barbosa-Cánovas G.V., Medina-Meza I., Candoğan K., Bermúdez-Aguirre D. Advanced retorting, microwave assisted thermal sterilization (MATS), and pressure assisted thermal sterilization (PATS) to process meat products. Meat Sci. 2014;98:420–434. doi: 10.1016/j.meatsci.2014.06.027. PubMed DOI

Yang B., Zhang Y., Jiang S., Lu J., Lin L. Effects of different cooking methods on the edible quality of crayfish (Procambarus clarkii) meat. Food Chem. Adv. 2023;2:100168.

Xie Q., Xu B., Xu Y., Yao Z., Zhu B., Li X., Sun Y. Effects of different thermal treatment temperatures on volatile flavour compounds of water-boiled duck after packaging. LWT. 2022;154:112625. doi: 10.1016/j.lwt.2021.112625. DOI

Microbiology of the Food Chain–Horizontal Method for the Enumeration of Microorganisms–Part 1: Colony Count at 30 °C by the Pour Plate Technique. International Organization for Standardization; Geneva, Switzerland: 2013.

Harrigan W.F. Laboratory Methods in Food Microbiology. 3rd ed. Academic Press; Amsterdam, The Netherlands: 1998.

Microbiology of Food and Animal Feeding Stuffs–Horizontal Method for the Enumeration of Yeasts and Moulds–Part 1: Colony Count Technique in Products with Water Activity Grater Than 0.95. International Organization for Standardization; Geneva, Switzerland: 2008.

Meat and Meat Products–Determination of Moisture Content (Reference Method) International Organization for Standardization; Geneva, Switzerland: 1997.

Food and Feed Products–General Guidelines for the Determination of Nitrogen by the Kjeldahl Method. International Organization for Standardization; Geneva, Switzerland: 2009.

Meat and Meat Products–Determination of Total Fat Content. International Organization for Standardization; Geneva, Switzerland: 1973.

Buňka F., Hrabě J., Kráčmar S. The effect of sterilisation on amino acid contents in processed cheese. Int. Dairy. J. 2004;14:829–831. doi: 10.1016/j.idairyj.2004.02.008. DOI

Kristensen D., Skibsted L.H. Comparison of three methods based on electron spin resonance spectrometry for evaluation of oxidative stability of processed cheese. J. Agric. Food Chem. 1999;47:3099–3104. doi: 10.1021/jf981396p. PubMed DOI

Jooyandeh H. Effect of fermented whey protein concentrate on texture of Iranian white cheese. J. Texture Stud. 2009;40:497–510. doi: 10.1111/j.1745-4603.2009.00194.x. DOI

Cheese–Determination of Rheological Properties by Uniaxial Compression at Constant Displacement Rate. International Organization for Standardization; Geneva, Switzerland: 2006.

Al-Hilphy A.R., Ali H.I., Al-IEssa S.A., Gavahian M., Mousavi-Khaneghah A. Assessing compositional and quality parameters of unconcentrated and refractive window concentrated milk based on color components. Dairy. 2022;3:400–412. doi: 10.3390/dairy3020030. DOI

Sensory Analysis–General Guidelines for the Selection, Training and Monitoring of Selected Assessors and Expert Sensory Assessors. International Organization for Standardization; Geneva, Switzerland: 2012.

Sensory Analysis–General Guidance for the Design of Test Rooms. International Organization for Standardization; Geneva, Switzerland: 2007.

Estévez M., Ventanas S., Cava R. Physicochemical properties and oxidative stability of liver pâté as affected by fat content. Food Chem. 2005;92:449–457. doi: 10.1016/j.foodchem.2004.08.014. DOI

Delgado-Pando G., Cofrades S., Ruiz-Capillas C., Triki M., Jiménez-Colmenero F. Low-fat pork liver pâtés enriched with n-3 PUFA/konjac gel: Dynamic rheological properties and technological behavior during chill storage. Meat Sci. 2012;92:44–52. doi: 10.1016/j.meatsci.2012.04.002. PubMed DOI

Friedman M. Food browning and its prevention. J. Agric. Food Chem. 1996;44:632–653. doi: 10.1021/jf950394r. DOI

Medina-Meza I.G., Barnaba C., Barbosa-Cánovas G.V. Effects of high pressure processing on lipid oxidation: A review. Innov. Food Sci. Emerg. 2014;22:1–10. doi: 10.1016/j.ifset.2013.10.012. DOI

Duranton F., Marée E., Simonin H., Chéret R., de Lamballerie M. Effect of high pressure–high temperature process on meat product quality. High. Press. Res. 2011;31:163–167. doi: 10.1080/08957959.2010.541242. DOI

Steen L., Fraeye I., De Mey E., Goemaere O., Paelinck H., Foubert I. Effect of Salt and Liver/Fat Ratio on Viscoelastic Properties of Liver Paste and Its Intermediates. Food Bioprocess. Technol. 2014;7:496–505. doi: 10.1007/s11947-012-1038-8. DOI

Kumar Y., Tyagi S.K., Vishwakarma R.K., Kalia A. Textural, microstructural, and dynamic rheological properties of low-fat meat emulsion containing aloe gel as potential fat replacer. Int. J. Food Prop. 2017;20:S1132–S1144. doi: 10.1080/10942912.2017.1336721. DOI

Niu L., Yu H., Zhang L., Zhao Q., Lai K., Liu Y., Huang Y. Advanced glycation end-products in raw and commercially sterilized pork tenderloin and offal. J. Food Compos. Anal. 2022;112:104681. doi: 10.1016/j.jfca.2022.104681. DOI

Studies on Food Physical Characterization