Multicompartment lipid cubic nanoparticles with high protein upload: millisecond dynamics of formation
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24742149
DOI
10.1021/nn5012946
Knihovny.cz E-zdroje
- MeSH
- biomimetika MeSH
- kapalné krystaly MeSH
- kinetika MeSH
- lidé MeSH
- lipidové dvojvrstvy chemie MeSH
- lipidy chemie MeSH
- maloúhlový rozptyl MeSH
- membrány umělé MeSH
- mozkový neurotrofický faktor chemie MeSH
- nanočástice chemie MeSH
- nanotechnologie metody MeSH
- neurotrofní faktory chemie MeSH
- proteiny chemie MeSH
- rekombinantní proteiny chemie MeSH
- transmisní elektronová mikroskopie MeSH
- vazba proteinů MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- BDNF protein, human MeSH Prohlížeč
- lipidové dvojvrstvy MeSH
- lipidy MeSH
- membrány umělé MeSH
- mozkový neurotrofický faktor MeSH
- neurotrofní faktory MeSH
- proteiny MeSH
- rekombinantní proteiny MeSH
Membrane shapes, produced by dynamically assembled lipid/protein architectures, are crucial for both physiological functions and the design of therapeutic nanotechnologies. Here we investigate the dynamics of lipid membrane-neurotrophic BDNF protein complexes formation and ordering in nanoparticles, with the purpose of innovation in nanostructure-based neuroprotection and biomimetic nanoarchitectonics. The kinetic pathway of membrane states associated with rapidly occurring nonequilibrium self-assembled lipid/protein nanoarchitectures was determined by millisecond time-resolved small-angle X-ray scattering (SAXS) at high resolution. The neurotrophin binding and millisecond trafficking along the flexible membranes induced an unusual overlay of channel-network architectures including two coexisting cubic lattices epitaxially connected to lamellar membrane stacks. These time-resolved membrane processes, involving intercalation of discrete stiff proteins in continuous soft membranes, evidence stepwise curvature control mechanisms. The obtained three-phase liquid-crystalline nanoparticles of neurotrophic composition put forward important advancements in multicompartment soft-matter nanostructure design.
Citace poskytuje Crossref.org
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