Multicompartment lipid cubic nanoparticles with high protein upload: millisecond dynamics of formation
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24742149
DOI
10.1021/nn5012946
Knihovny.cz E-resources
- MeSH
- Biomimetics MeSH
- Liquid Crystals MeSH
- Kinetics MeSH
- Humans MeSH
- Lipid Bilayers chemistry MeSH
- Lipids chemistry MeSH
- Scattering, Small Angle MeSH
- Membranes, Artificial MeSH
- Brain-Derived Neurotrophic Factor chemistry MeSH
- Nanoparticles chemistry MeSH
- Nanotechnology methods MeSH
- Nerve Growth Factors chemistry MeSH
- Proteins chemistry MeSH
- Recombinant Proteins chemistry MeSH
- Microscopy, Electron, Transmission MeSH
- Protein Binding MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- BDNF protein, human MeSH Browser
- Lipid Bilayers MeSH
- Lipids MeSH
- Membranes, Artificial MeSH
- Brain-Derived Neurotrophic Factor MeSH
- Nerve Growth Factors MeSH
- Proteins MeSH
- Recombinant Proteins MeSH
Membrane shapes, produced by dynamically assembled lipid/protein architectures, are crucial for both physiological functions and the design of therapeutic nanotechnologies. Here we investigate the dynamics of lipid membrane-neurotrophic BDNF protein complexes formation and ordering in nanoparticles, with the purpose of innovation in nanostructure-based neuroprotection and biomimetic nanoarchitectonics. The kinetic pathway of membrane states associated with rapidly occurring nonequilibrium self-assembled lipid/protein nanoarchitectures was determined by millisecond time-resolved small-angle X-ray scattering (SAXS) at high resolution. The neurotrophin binding and millisecond trafficking along the flexible membranes induced an unusual overlay of channel-network architectures including two coexisting cubic lattices epitaxially connected to lamellar membrane stacks. These time-resolved membrane processes, involving intercalation of discrete stiff proteins in continuous soft membranes, evidence stepwise curvature control mechanisms. The obtained three-phase liquid-crystalline nanoparticles of neurotrophic composition put forward important advancements in multicompartment soft-matter nanostructure design.
References provided by Crossref.org
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