Apoptosis induction in human hepatoma cell line HepG2 cells by trans- Anethole via activation of mitochondria-mediated apoptotic pathways
Jazyk angličtina Země Francie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
37531780
DOI
10.1016/j.biopha.2023.115236
PII: S0753-3322(23)01027-2
Knihovny.cz E-zdroje
- Klíčová slova
- Antiproliferative, Apoptosis, DNA nicking, HepG2, Mitochondrial membrane potential (MMP), Reactive oxygen species (ROS),
- MeSH
- apoptóza MeSH
- buňky Hep G2 MeSH
- HeLa buňky MeSH
- hepatocelulární karcinom * farmakoterapie metabolismus MeSH
- lidé MeSH
- membránový potenciál mitochondrií MeSH
- mitochondrie metabolismus MeSH
- nádorový supresorový protein p53 metabolismus MeSH
- nádory jater * farmakoterapie metabolismus MeSH
- reaktivní formy kyslíku metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- anethole MeSH Prohlížeč
- nádorový supresorový protein p53 MeSH
- reaktivní formy kyslíku MeSH
trans-Anethole a valuable compound derived from star anise widely used by ethnic tribals to manage numerous human diseases. In this study antiproliferative activities of trans-Anethole towards human liver cancer (HepG2), cervical cancer (HeLa) and breast cancer (MCF-7) cells were explored. trans-Anethole showed free radical scavenging potential as assessed by DNA nicking assay. trans-Anethole exhibited strong antiproliferative potential towards HepG2 cells compared to other cell lines. trans-Anethole strongly induced apoptosis in HepG2 cells by significantly upregulating the protein expressions of p53, Caspase-3 and Caspase-9 were assessed by western blotting analysis which highlighted apoptosis-inducing capacity of trans-Anethole against HepG2 cells. Rt-qPCR analysis revealed that trans- Anethole upregulated p53, caspase - 3 and - 9 in comparison to untreated HepG2 cancer cells. Moreover, trans-Anethole provoked the generation of ROS and disruption of MMP. Our research suggests that trans-Anethole may have a significant anticancer therapeutic potential for treating liver cancer.
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