BACKGROUND: Hydrogen is a potent antioxidant agent that can easily be administered by inhalation. The aim of the study was to evaluate whether hydrogen protects the endothelial glycocalyx layer after successful cardiopulmonary resuscitation (CPR). METHODS: Fourteen anesthetized pigs underwent CPR after induced ventricular fibrillation. During CPR and return of spontaneous circulation, 2% hydrogen gas was administered to seven pigs (hydrogen group) and seven constituted a control group. Biochemistry and sublingual microcirculation were assessed at baseline, during CPR, at the 15th, 30th, 60th, 120th minute. RESULTS: All seven subjects from the hydrogen group and six subjects in the control group were successfully resuscitated after 6-10 minutes. At baseline, there were no statistically significant differences in examined variables. After the CPR, blood pH, base excess, and lactate showed significantly smaller deterioration in the hydrogen group than in the control group. By contrast, plasma syndecan-1 and the measured variables obtained via sublingual microcirculation did not change after the CPR; and were virtually identical between the two groups. CONCLUSION: In pigs, hydrogen gas inhalation during CPR and post-resuscitation care was associated with less pronounced metabolic acidosis compared to controls. However, we could not find evidence of injury to the endothelium or glycocalyx in any studied groups.

1st Department of Anaesthesiology and Intensive Therapy Medical University of Lublin Lublin Poland

Constantine the Philosopher University in Nitra Faculty of Social Sciences and Health Care Nitra Slovak Republic

Department of Anesthesia Pain Management and Perioperative Medicine Dalhousie University Halifax NS Canada

Department of Anesthesiology and Resuscitation University Hospital Kralovske Vinohrady Prague Czech Republic

Department of Anesthesiology Perioperative Medicine and Intensive Care Hospital Bory Bratislava Slovak Republic

Department of Anesthesiology Perioperative Medicine and Intensive Care Medicine University of J E Purkyne in Usti nad Labem Masaryk Hospital in Usti nad Labem Usti nad Labem Czech Republic

Department of Anesthesiology Resuscitation and Intensive Care Medicine University Hospital Hradec Kralove Hradec Kralove Czech Republic

Department of Clinical Biochemistry and Diagnostics University Hospital Hradec Kralove Hradec Kralove Czech Republic

Department of Emergency Medicine Hospital Bory Bratislava Slovak Republic

Department of Microbiology and Immunology Dalhousie University Halifax NS Canada

Department of Pharmacology Dalhousie University Halifax NS Canada

Department of Physiology and Biophysics Dalhousie University Halifax NS Canada

Department of Urology University Hospital Hradec Kralove Hradec Kralove Czech Republic

Faculty of Health Studies Technical University in Liberec Liberec Czech Republic

Faculty of Medicine in Hradec Kralove Charles University Prague Czech Republic

Faculty of Military Health Sciences University of Defence Brno Czech Republic

Institute of Postgradual Education in Healthcare Prague Czech Republic

International Fluid Academy Lovenjoel Belgium

Karolinska Institutet at Danderyds Hospital Stockholm Sweden

Medical Data Management Medaman Pas Geel Belgium

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