The aim of our study was to describe the impact of collagen in the gel and dry state to various doses of electron beam radiation (1, 10 and 25 kGy) which are using for food processing and sterilization. The changes in the chemical compositions (water, amino acids, lipids, glycosaminoglycans) were analyzed and the changes in the structure (triple-helix or β-sheet, the integrity of the collagen) were assessed. Subsequently, the impact of the applied doses on the mechanical properties, stability in the enzymatic environment, swelling and morphology were determined. The irradiated gels evinced enhanced degrees of cross-linking with only partial degradation. Nevertheless, an increase was observed in their stability manifested via a higher degree of resistance to the enzymatic environment, a reduction in swelling and, in terms of the mechanical behaviour, an approximation to the non-linear behavior of native tissues. In contrast, irradiation in the dry state exerted a somewhat negative impact on the observed properties and was manifested mainly via the scission of the collagen molecule and via a lower degree of stability in the aqueous and enzymatic environments. Neither the chemical composition nor the morphology was affected by irradiation.

Department of Composites and Carbon Materials Institute of Rock Structure and Mechanics Czech Academy of Sciences 182 09 Prague 8 Czech Republic

Department of Composites and Carbon Materials Institute of Rock Structure and Mechanics Czech Academy of Sciences 182 09 Prague 8 Czech Republic; Department of Mechanics Biomechanics and Mechatronics Faculty of Mechanical Engineering Czech Technical University Prague 166 07 Prague 6 Czech Republic

Department of Mechanics Biomechanics and Mechatronics Faculty of Mechanical Engineering Czech Technical University Prague 166 07 Prague 6 Czech Republic

Department of Polymers University of Chemistry and Technology Prague 166 28 Prague 6 Czech Republic

Food Research Institute Prague 102 00 Prague 10 Czech Republic

Nuclear Physics Institute CAS v v i 250 68 Řež Czech Republic

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