Somatic Genomic and Transcriptomic Characterization of Primary Ovarian Serous Borderline Tumors and Low-Grade Serous Carcinomas
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
38280423
DOI
10.1016/j.jmoldx.2023.12.004
PII: S1525-1578(24)00007-2
Knihovny.cz E-zdroje
- MeSH
- azosloučeniny * MeSH
- genomika MeSH
- lidé MeSH
- mutace MeSH
- nádory vaječníků * diagnóza genetika MeSH
- protoonkogenní proteiny B-Raf genetika MeSH
- RNA MeSH
- serózní cystadenokarcinom * diagnóza genetika MeSH
- stanovení celkové genové exprese MeSH
- stupeň nádoru MeSH
- thiolesterasa ubikvitinu genetika MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- azosloučeniny * MeSH
- Martius scarlet blue trichrome MeSH Prohlížeč
- protoonkogenní proteiny B-Raf MeSH
- RNA MeSH
- thiolesterasa ubikvitinu MeSH
- USP9X protein, human MeSH Prohlížeč
Low-grade serous carcinoma (LGSC) may develop from serous borderline tumor (SBT) tissue, where the micropapillary type (mSBT) presents the highest risk for progression. The sensitivity of LGSC to standard chemotherapy is limited, so alternative therapeutic approaches, including targeted treatment, are needed. However, knowledge about the molecular landscape of LGSC and mSBT is limited. A sample set of 137 pathologically well-defined cases (LGSC, 97; mSBT, 40) was analyzed using capture DNA next-generation sequencing (727 genes) and RNA next-generation sequencing (147 genes) to show the landscape of somatic mutations, gene fusions, expression pattern, and prognostic and predictive relevance. Class 4/5 mutations in the main driver genes (KRAS, BRAF, NRAS, ERBB2, USP9X) were detected in 48% (14/29) of mSBT cases and 63% (47/75) of LGSC cases. The USP9X mutation was detected in only 17% of LGSC cases. RNA next-generation sequencing revealed gene fusions in 6 of 64 LGSC cases (9%) and 2 of 33 mSBT cases (9%), and a heterogeneous expression profile across LGSC and mSBT. No molecular characteristics were associated with greater survival. The somatic genomic and transcriptomic profiles of 35 mSBT and 85 LGSC cases are compared for the first time. Candidate oncogenic gene fusions involving BRAF, FGFR2, or NF1 as a fusion partner were identified. Molecular testing of LGSC may be used in clinical practice to reveal therapeutically significant targets.
Department of Oncological Pathology Masaryk Memorial Cancer Institute Brno Czech Republic
Department of Pathology Bulovka Hospital Prague Czech Republic
Department of Pathology Faculty of Medicine University of Debrecen Debrecen Hungary
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