The correlation between human seminal plasma sialoproteins and ejaculate parameters
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
38574922
DOI
10.1016/j.ijbiomac.2024.131341
PII: S0141-8130(24)02146-9
Knihovny.cz E-resources
- Keywords
- Anti-sperm antibodies, Apoptosis, Ejaculate quality, Glycoprotein, Human,
- MeSH
- Semen Analysis methods MeSH
- Apoptosis MeSH
- Ejaculation MeSH
- Glycodelin metabolism MeSH
- Glycoproteins metabolism MeSH
- Clusterin metabolism MeSH
- Sialic Acids metabolism MeSH
- Lactoferrin metabolism MeSH
- Lectins metabolism chemistry MeSH
- Humans MeSH
- Sperm Motility MeSH
- Seminal Plasma Proteins metabolism MeSH
- Seminal Vesicle Secretory Proteins metabolism MeSH
- Semen * metabolism chemistry MeSH
- Spermatozoa * metabolism MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Glycodelin MeSH
- Glycoproteins MeSH
- Clusterin MeSH
- Sialic Acids MeSH
- Lactoferrin MeSH
- Lectins MeSH
- PAEP protein, human MeSH Browser
- Seminal Plasma Proteins MeSH
- Seminal Vesicle Secretory Proteins MeSH
- seminal vesicle-specific antigen MeSH Browser
Sialic acids are negatively charged carbohydrates that are components of saccharide chains covalently linked to macromolecules. Sialylated glycoproteins are important for most biological processes, including reproduction, where they are associated with spermatogenesis, sperm motility, immune responses, and fertilization. Changes in the glycoprotein profile or sialylation in glycoproteins are likely to affect the quality of ejaculate. The aim of this study was to determine differences in the degree of sialylation between normozoospermic ejaculates and ejaculates with a pathological spermiogram using two lectins, Sambucus nigra (SNA) and Maackia amurensis (MAL II/MAA) recognizing α-2,6 or α-2,3 linkage of Sia to galactosyl residues. Our results show a close relationship between seminal plasma (SP) sialoproteins and the presence of anti-sperm antibodies in the ejaculate, apoptotic spermatozoa, and ejaculate quality. Using mass spectrometry, we identified SP sialoproteins such as, semenogelins, glycodelin, prolactin-inducible protein, lactotransferrin, and clusterin that are associated with spermatozoa and contribute to the modulation of the immune response and sperm apoptosis. Our findings suggest a correlation between the degree of SP glycoprotein sialylation and the existence of possible pathological states of spermatozoa and reproductive organs. Glycoproteins sialylation represents a potential parameter reflecting the overall quality of ejaculate and could potentially be utilised in diagnostics.
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