Thermal evaporation as sample preparation for silver-assisted laser desorption/ionization mass spectrometry imaging of cholesterol in amyloid tissues
Jazyk angličtina Země Velká Británie, Anglie Médium electronic
Typ dokumentu časopisecké články
PubMed
38630503
DOI
10.1039/d4an00181h
Knihovny.cz E-zdroje
- MeSH
- Alzheimerova nemoc metabolismus patologie MeSH
- amyloidní plaky MeSH
- amyloidóza metabolismus patologie MeSH
- cholesterol * analýza chemie MeSH
- lidé MeSH
- mozek metabolismus diagnostické zobrazování MeSH
- myokard metabolismus chemie patologie MeSH
- myši transgenní MeSH
- myši MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice * metody MeSH
- stříbro * chemie MeSH
- teplota MeSH
- volatilizace MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- cholesterol * MeSH
- stříbro * MeSH
Cholesterol plays an important biological role in the body, and its disruption in homeostasis and synthesis has been implicated in several diseases. Mapping the locations of cholesterol is crucial for gaining a better understanding of these conditions. Silver deposition has proven to be an effective method for analyzing cholesterol using mass spectrometry imaging (MSI). We optimized and evaluated thermal evaporation as an alternative deposition technique to sputtering for silver deposition in MSI of cholesterol. A silver layer with a thickness of 6 nm provided an optimal combination of cholesterol signal intensity and mass resolution. The deposition of an ultrathin nanofilm of silver enabled high-resolution MSI with a pixel size of 10 μm. We used this optimized method to visualize the distribution of cholesterol in the senile plaques in the brains of APP/PS1 mice, a model that resembles Alzheimer's disease pathology. We found that cholesterol was evenly distributed across the frontal cortex tissue, with no evidence of plaque-like accumulation. Additionally, we investigated the presence and distribution of cholesterol in myocardial sections of a human heart affected by wild-type ATTR amyloidosis. We identified the presence of cholesterol in areas with amyloid deposition, but complete colocalization was not observed.
Citace poskytuje Crossref.org