The research is focused on the quantitative evaluation of the flaxseed (Linum usitatissimum L.) proteome at the level of seed cake (SC), fine flour-sieved a fraction below 250 µm (FF)-and protein concentrate (PC). The evaluation was performed on three oilseed flax cultivars (Agriol, Raciol, and Libra) with different levels of α-linolenic acid content using LC-MS/MS (shotgun proteomics) analysis, which was finalized by database searching using the NCBI protein database for Linum usitatissimum and related species. A total of 2560 protein groups (PGs) were identified, and their relative abundance was calculated. A set of 33 quantitatively most significant PGs was selected for further characterization. The selected PGs were divided into four classes-seed storage proteins (11S globulins and conlinins), oleosins, defense- and stress-related proteins, and other major proteins (mainly including enzymes). Seed storage proteins were found to be the most abundant proteins. Specifically, 11S globulins accounted for 41-44% of SC proteins, 40-46% of FF proteins, and 72-84% of PC proteins, depending on the cultivar. Conlinins (2S albumins) were the most abundant in FF, ranging from 10 to 13% (depending on cultivar). The second most important class from the point of relative abundance was oleosins, which were represented in SC and FF in the range of 2.1-3.8%, but only 0.36-1.20% in PC. Surprisingly, a relatively high abundance of chitinase was found in flax products as a protein related to defence and stress reactions.

Department of Dairy Fat and Cosmetics University of Chemistry and Technology Technická 5 166 28 Prague Czech Republic

Department of Food Biotechnology and Agricultural Products Quality Faculty of Agriculture and Technology University of South Bohemia Studentská 1668 370 05 České Budějovice Czech Republic

Department of Legumes and Technical Crops Agritec Plant Research Ltd Zemědělská 2520 16 787 01 Šumperk Czech Republic

Department of Plant Production Faculty of Agriculture and Technology University of South Bohemia Na Sádkách 1780 370 05 České Budějovice Czech Republic

Mendel Centre of Plant Genomics and Proteomics Central European Institute of Technology Masaryk University Kamenice 753 5 625 00 Brno Czech Republic

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