Oilseed cakes are produced as a by-product of oil pressing and are mostly used as feed. Their use for human consumption is due to the functional properties and benefits for human health. Herein, oilseed cake flours of eight species (flax, hemp, milk thistle, poppy, pumpkin, rapeseed, safflower, sunflower) were sieved into fractions above (A250) and below (B250) 250 µm. The chemical composition, SDS-PAGE profiles, colour, functional properties and antioxidant activities of these flours were evaluated. The B250 fractions were evaluated as being protein and ash rich, reaching crude protein and ash content ranging from 31.78% (milk thistle) to 57.47% (pumpkin) and from 5.0% (flax) to 11.19% (poppy), respectively. A high content of carbohydrates was found in the flours of hemp, milk thistle and safflower with a significant increase for the A250 fraction, with a subsequent relation to a high water holding capacity (WHC) for the A250 fraction (flax, poppy, pumpkin and sunflower). The A250 milk thistle flour was found to have the richest in polyphenols content (TPC) (40.89 mg GAE/g), with the highest antioxidant activity using an ABTS•+ assay (101.95 mg AAE/g). The A250 fraction for all the species exhibited lower lightness than the B250 fraction. The obtained results indicate that sieving oilseed flour with the aim to prepare flours with specific functional characteristics and composition is efficient only in combination with a particular species.

Department of Dairy Fat and Cosmetics University of Chemistry and Technology 166 28 Prague Czech Republic

Department of Food Biotechnology and Agricultural Products Quality Faculty of Agriculture University of South Bohemia 370 05 České Budějovice Czech Republic

Department of Legumes and Technical Crops Agritec Plant Research Ltd 787 01 Šumperk Czech Republic

Department of Plant Production Faculty of Agriculture University of South Bohemia 370 05 České Budějovice Czech Republic

Institute of Agroecology and Plant Production Wrocław University of Environmental and Life Sciences 50 363 Wrocław Poland

Mendel Centre of Plant Genomics and Proteomics Central European Institute of Technology Masaryk University 625 00 Brno Czech Republic

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