poppy Dotaz Zobrazit nápovědu
Suspenzné kultúry maku siateho (Papaver somniferum L.) sa permeabilizovali Tweenom 80 a imo- bilizovali glutaraldehydom. pH optimum invertázy v imobilizovaných bunkách je 4,6 a tepelné optimum je pri 50 °C. Enzýmová reakcia má lineárny priebeh 5 hodín a dosahuje 69 % konverzie. Imobilizované bunky majú vysokú invertázovú aktivitu a výhodné mechanické vlastnosti.
Cell suspension culture of the opium poppy plant (Papaver somniferum L.) was permeabilized by Tween 80 and immobilized by glutaraldehyde. The pH optimum of the immobilized cells' invertase is 4.6 and the temperature optimum is 50 °C. The enzyme hydrolysis was linear for 5 h, reaching 69 % of conversion. The immobilized cells are characterized by a high invertase activity and showed convenient mechanical properties.
Suspenzné kultúry maku siateho (Papaver somniferum L.) sa permeabilizovali Tweenom 80 a imo-bilizovali glutaraldehydom. pH optimum L-alanínaminopeptidázy (L-Ala-AP), L-tyrozínaminopep-tidázy (L-Tyr-AP) a L-arginínaminopeptidázy (L-Arg-AP) v natívnych a v imobilizovaných bunkáchje 8,0; 7,0 a 7,8 resp. V bunkách imobilizovaných glutaraldehydom je v porovnaní s natívnymibunkami veľmi nízka aktivita sledovaných enzýmov. Pri imobilizácii aminopeptidáz v bunkáchmaku sa úspešne aplikoval alginátový gél.
Suspension cultures of the poppy plant (Papaver somniferum L.) were permeated with Tween 80and immobilized with glutaraldehyde. The values of optimum pH of L-alanine aminopeptidase(L-Ala-AP), L-tyrosine aminopeptidase (L-Tyr-AP) and L-arginine aminopeptidase (L-Arg-AP) innative and immobilized cells are 8.0; 7.0 and 7.8, respectively. In the cells immobilized withglutaraldehyde, the activity of the enzymes under study is very low in comparison with native cells.In the immobilization of amino peptidases, alginate gel was successfully applied.
- MeSH
- algináty MeSH
- aminopeptidasy MeSH
- farmaceutická chemie MeSH
- glutaraldehyd MeSH
- kultivované buňky MeSH
- lidé MeSH
- Papaver MeSH
- Check Tag
- lidé MeSH
