Cytokinin oxidase/dehydrogenase inhibitors: progress towards agricultural practice
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu časopisecké články
Grantová podpora
Czech Science Foundation
PubMed
38776394
DOI
10.1093/jxb/erae239
PII: 7679848
Knihovny.cz E-zdroje
- Klíčová slova
- Agriculture, Arabidopsis, CKX inhibitor, biostimulant, biotechnology, cytokinin, cytokinin oxidase/dehydrogenase, diphenylurea, nutrient use efficiency, oilseed rape,
- MeSH
- Arabidopsis * účinky léků genetika MeSH
- Brassica napus genetika účinky léků MeSH
- cytokininy metabolismus MeSH
- inhibitory enzymů farmakologie MeSH
- kukuřice setá účinky léků genetika růst a vývoj MeSH
- oxidoreduktasy * metabolismus genetika MeSH
- rostlinné proteiny metabolismus genetika chemie MeSH
- vztahy mezi strukturou a aktivitou MeSH
- zemědělství MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- cytokinin oxidase MeSH Prohlížeč
- cytokininy MeSH
- inhibitory enzymů MeSH
- oxidoreduktasy * MeSH
- rostlinné proteiny MeSH
Cytokinin oxidase/dehydrogenase (CKX) inhibitors reduce the degradation of cytokinins in plants and thereby may improve the efficiency of agriculture and plant tissue culture-based practices. Here, we report a synthesis and structure-activity relationship study of novel urea derivatives concerning their CKX inhibitory activity. The most active compounds showed sub-nanomolar IC50 values with maize ZmCKX1, the lowest value yet documented. Other CKX isoforms of maize and Arabidopsis were also inhibited very effectively. The binding mode of four compounds was characterized based on high-resolution crystal complex structures. Using the soil nematode Caenorhabditis elegans, and human skin fibroblasts, key CKX inhibitors with low toxicity were identified. These compounds enhanced the shoot regeneration of Lobelia, Drosera, and Plectranthus, as well as the growth of Arabidopsis and Brassica napus. At the same time, a key compound (identified as 82) activated a cytokinin primary response gene, ARR5:GUS, and a cytokinin sensor, TCSv2:GUS, without activating the Arabidopsis cytokinin receptors AHK3 and AHK4. This strongly implies that the effect of compound 82 is due to the up-regulation of cytokinin signalling. Overall, this study identifies highly effective and easily prepared CKX inhibitors with a low risk of environmental toxicity for further investigation of their potential in agriculture and biotechnology.
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