Long circulating liposomal platform utilizing hydrophilic polymer-based surface modification: preparation, characterisation, and biological evaluation
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
39004290
DOI
10.1016/j.ijpharm.2024.124465
PII: S0378-5173(24)00699-9
Knihovny.cz E-resources
- Keywords
- Complement activation, Drug delivery system, HPMA copolymer, Long-circulating liposomes, Stealth liposomes,
- MeSH
- Acrylamides chemistry MeSH
- Complement Activation drug effects MeSH
- Cholesterol chemistry blood MeSH
- Hydrophobic and Hydrophilic Interactions * MeSH
- Rabbits MeSH
- Drug Delivery Systems MeSH
- Humans MeSH
- Liposomes * MeSH
- Mice MeSH
- Polyethylene Glycols * chemistry MeSH
- Polymers chemistry MeSH
- Surface Properties * MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Humans MeSH
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Acrylamides MeSH
- Cholesterol MeSH
- Liposomes * MeSH
- Polyethylene Glycols * MeSH
- Polymers MeSH
Liposomes are one of the most important drug delivery vectors, nowadays used in clinics. In general, polyethylene glycol (PEG) is used to ensure the stealth properties of the liposomes. Here, we have employed hydrophilic, biocompatible and highly non-fouling N-(2-hydroxypropyl) methacrylamide (HPMA)-based copolymers containing hydrophobic cholesterol anchors for the surface modification of liposomes, which were prepared by the method of lipid film hydration and extrusion through 100 nm polycarbonate filters. Efficient surface modification of liposomes was confirmed by transmission electron microscopy, atomic force microscopy, and gradient ultracentrifugation. The ability of long-term circulation in the vascular bed was demonstrated in rabbits after i.v. application of fluorescently labelled liposomes. Compared to PEGylated liposomes, HPMA-based copolymer-modified liposomes did not induce specific antibody formation and did not activate murine and human complement. Compared with PEGylated liposomes, HPMA-based copolymer-modified liposomes showed a better long-circulating effect after repeated administration. HPMA-based copolymer-modified liposomes thus represent suitable new candidates for a generation of safer and improved liposomal drug delivery platforms.
Department of Medicine Department of Biochemistry Masaryk University Brno Czech Republic
ICRC International Clinical Research Center St Anne's University Hospital Brno Czech Republic
Institute of Physics Czech Academy of Sciences Na Slovance 1999 2 182 00 Prague 8 Czech Republic
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