We present a genome assembly from an individual female Spicauda simplicius (the Plain Longtail butterfly; Arthropoda; Insecta; Lepidoptera; Hesperiidae). The genome sequence is 610.1 megabases in span. Most of the assembly is scaffolded into 32 chromosomal pseudomolecules, including the Z and W sex chromosomes. The mitochondrial genome has also been assembled and is 15.54 kilobases in length. Gene annotation of this assembly on Ensembl identified 18,506 protein coding genes.

Biology Centre of the Czech Academy of Sciences Institute of Entomology České Budějovice Czech Republic

Faculty of Science University of South Bohemia České Budějovice Czech Republic

Wellcome Sanger Institute Hinxton England UK

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