Streptococcus suis strains with novel and previously undescribed capsular loci circulate in Europe
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
39340873
DOI
10.1016/j.vetmic.2024.110265
PII: S0378-1135(24)00287-6
Knihovny.cz E-zdroje
- Klíčová slova
- NCL, Novel cps loci, Streptococcus suis, cps locus,
- MeSH
- bakteriální pouzdra * genetika MeSH
- fylogeneze * MeSH
- nemoci prasat * mikrobiologie epidemiologie MeSH
- prasata MeSH
- séroskupina * MeSH
- sérotypizace MeSH
- Streptococcus suis * genetika patogenita klasifikace izolace a purifikace MeSH
- streptokokové infekce * veterinární mikrobiologie MeSH
- virulence genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
- Evropa epidemiologie MeSH
Streptococcus suis (S. suis) causes serious diseases in pigs, and certain serotypes also pose a risk to humans. The expression of capsular polysaccharides (CPS) is considered an important virulence property of the pathogen. Recently, some serotypes have been reclassified as other organisms, while novel S. suis serotypes are being described. Although the CPS can be typed by serological methods using antisera, the presence of unique sequences for each capsular polysaccharide synthesis locus (cps locus) enables convenient PCR-based serotyping. In this study, we characterized 33 non-serotypeable S. suis strains obtained from diseased pigs in the Czech Republic by sequencing and analyzing the cps locus. Phylogenetic analysis of cpn60 confirmed that all isolates belong to the S. suis species. Four isolates had cps loci similar to the previously described reference S. suis serotypes. Eleven isolates were classified as recently described novel cps loci (NCLs). Nine isolates had substitutions, insertions and/or deletions in their cps loci and showed only partial similarity to the already described NCLs. Another eight isolates had previously undescribed cps locus structures and were proposed as novel NCLs. One isolate had lost the genes encoding capsule biosynthesis. Only four sequence types (ST) had two isolates each; the rest had unique STs. Two isolates harbored the classical virulence associated genes (VAGs) mrp and sly. Another isolate had only the mrp gene, while a different isolate harbored only the sly gene. This study provides insight into untypeable isolates in the Czech Republic, highlighting the genetic diversity and potential for novel serotype identification.
State Veterinary Institute Jihlava Jihlava 586 01 Czech Republic
Citace poskytuje Crossref.org
Genomic re-evaluation of clinical isolates reveals a structured Streptococcus suis complex
