The perinucleolar compartment and the oncogenic super-enhancers are part of the same phase-separated structure filled with phosphatidylinositol 4,5-bisphosphate and long non-coding RNA HANR
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, přehledy
PubMed
39648081
DOI
10.1016/j.jbior.2024.101069
PII: S2212-4926(24)00057-5
Knihovny.cz E-zdroje
- Klíčová slova
- HANR, Long non-coding RNA, Oncogenic super-enhancers, PIP2, PNC, Perinucleolar compartment, Phase separation, Phosphatidylinositol 4,5-bisphosphate,
- MeSH
- buněčné jadérko * metabolismus genetika MeSH
- fosfatidylinositol-4,5-difosfát * metabolismus genetika MeSH
- lidé MeSH
- nádory * genetika metabolismus patologie MeSH
- RNA dlouhá nekódující * genetika metabolismus MeSH
- zesilovače transkripce * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
- Názvy látek
- fosfatidylinositol-4,5-difosfát * MeSH
- RNA dlouhá nekódující * MeSH
The liquid-liquid phase separation in the cell nucleus regulates various processes such as gene regulation and transcription control, chromatin organization, and DNA repair. A plethora of proteins and RNAs contribute to the formation of biomolecular condensates and recently, several nuclear phosphoinositides were shown to be a part of these membrane-less complexes within the nucleus as well. Here we lipid-interacting RNA sequencing (LIPRNAseq) and confocal microscopy to uncover the RNA-binding capacity and localization of phosphatidylinositol 4,5 bisphosphate (PIP2). We discovered the consensus PIP2-binding AU-rich RNA motif and identified long non-coding RNA HANR (lncHANR) to colocalize with PIP2 in the proximity to the nucleolus in the perinucleolar compartment (PNC). Colocalization studies with different nuclear markers reveal that PIP2-HANR presence in the PNC correlates with oncogenic super-enhancers, and both PNC and oncogenic enhancers are part of the same structure. As lncHANR, PNC, and oncogenic super-enhancers are associated with cancer cell lines and tumors, we suggest that they can serve as interchangeable prognostic markers. Understanding of the interplay between lipid metabolism, and lncRNAs in subnuclear compartment phase separation can lead to future improvement in treatment strategies and personalized cancer management approaches.
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