Cryptosporidium mortiferum, a parasite transmitted by squirrels, is beginning to spread in Europe. C. mortiferum was detected in a symptomatic human. A 44-year-old man from the Czech Republic suffered from gastroenteritis characterized by abdominal pain, nausea, and loose stools. Molecular analyses confirmed the XIVaA20G2T1 subtype in the patient's stool. At the same time, the same subtype of C. mortiferum was detected in three red squirrels and two ground squirrels in the area where the patient lived. The intensity of the infection was significantly higher in the red squirrels that died, while the ground squirrels showed no symptoms. The results of the study indicate that red squirrels and ground squirrels are the reservoirs for the infection.

Czech Union of Nature Conservationists Animal Rescue Center Vlašim Czech Republic

Department of Biology and Medical Parasitology Wroclaw Medical University Wroclaw Poland

Department of Microbiological Sciences North Dakota State University Fargo ND USA

Faculty of Agriculture and Technology University of South Bohemia České Budějovice Czech Republic

Institute of Parasitology Biology Centre of the Czech Academy of Sciences České Budějovice Czech Republic

Institute of Public Health based in Ústí nad Labem National Reference Laboratory for the Diagnosis of Parasitic Intestinal Diseases Ústí nad Labem Czech Republic

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Nader JL, Mathers TC, Ward BJ, et al. . Evolutionary genomics of anthroponosis in Cryptosporidium. Nat Microbiol. 2019;4:826–36. PubMed

Jiang J, Alderisio KA, Xiao L.. Distribution of Cryptosporidium genotypes in storm event water samples from three watersheds in New York. Appl Environ Microbiol. 2005;71:4446–54. PubMed PMC

Lebbad M, Beser J, Insulander M, et al. . Unusual cryptosporidiosis cases in Swedish patients: extended molecular characterization of Cryptosporidium viatorum and Cryptosporidium chipmunk genotype I. Parasitology. 2013;140:1735–40. PubMed

Stenger BL, Clark ME, Kváč M, et al. . North American tree squirrels and ground squirrels with overlapping ranges host different Cryptosporidium species and genotypes. Infect Genet Evol. 2015;36:287–93. PubMed

Stensvold CR, Larsen TG, Grüttner J, et al. . Rodent-adapted infection in humans: Seven new cases and review of the literature. One Health. 2024;18:100682. PubMed PMC

Häkkänen T, Rimhanen-Finne R, Antikainen J, et al. . Molecular characteristics of spp. in human cases in five Finnish hospital districts during 2021: first findings of chipmunk genotype I) in Finland. Int J Parasitol. 2024;54:225–31. PubMed

Prediger J, Horčičková M, Hofmannová L, et al. . Native and introduced squirrels in Italy host different Cryptosporidium spp. Eur J Protistol. 2017;61:64–75. PubMed

Kváč M, Hofmannová L, Bertolino S, et al. . Natural infection with two genotypes of Cryptosporidium in red squirrels (Sciurus vulgaris) in Italy. Folia Parasitol. 2008;55:95–9. PubMed

Henriksen SA, Pohlenz JF.. Staining of cryptosporidia by a modified Ziehl-Neelsen technique. Acta Vet Scand. 1981;22:594–6. PubMed PMC

Sak B, Kváč M, Hanzlíková D, et al. . First report of Enterocytozoon bieneusi infection on a pig farm in the Czech Republic. Vet Parasitol. 2008;153:220–4. PubMed

Guo Y, Cebelinski E, Matusevich C, et al. . Subtyping novel zoonotic pathogen Cryptosporidium chipmunk genotype I. J Clin Microbiol. 2015;53:1648–54. PubMed PMC

Tůmová L, Ježková J, Prediger J, et al. . Cryptosporidium mortiferum n. sp. (Apicomplexa: Cryptosporidiidae), the species causing lethal cryptosporidiosis in Eurasian red squirrels (Sciurus vulgaris). Parasit Vectors. 2023;16:235. PubMed PMC

Prediger J, Ježková J, Holubová N, et al. . Cryptosporidium sciurinum n. sp. (Apicomplexa: Cryptosporidiidae) in Eurasian Red Squirrels (Sciurus vulgaris). Microorganisms. 2021;9:2050. PubMed PMC

Bujila I, Troell K, Fischerstrom K, et al. . Cryptosporidium chipmunk genotype I – An emerging cause of human cryptosporidiosis in Sweden. Infect Genet Evol. 2021;92:104895. PubMed