INTRODUCTION: Stem cells derived from adipose tissue are gaining popularity in the field of regenerative medicine due to their adaptability and clinical potential. Their rapid growth, ability to differentiate, and easy extraction with minimal complications make adipose-derived stem cells (ADSCs) a promising option for many treatments, particularly those targeting bone-related diseases. This study analyzed gene expression in canine ADSCs subjected to long-term culture and osteogenic differentiation. METHODS: ADSCs were isolated from discarded surgical waste and cultured for 14 days with and without differentiation media to assess osteogenic changes. RNA sequencing (RNA-seq) and bioinformatical analysis were performed to obtain comprehensive transcriptomic data. A total of 17793 genes were detected and GO enrichment analysis was performed on the differentially expressed genes to identify significantly up- and downregulated Biological Process (BP) GO terms across each comparison. RESULTS: The upregulation of apoptosis-regulating genes and genes related to circulatory system development suggest an induction of these processes, while the downregulation of neurogenesis and gliogenesis genes points to reciprocal regulation during osteogenic differentiation of canine ADSCs. DISCUSSION: These findings underscore the potential of ADSCs in bone regeneration and offer valuable insights for advancing tissue engineering, however further studies, including proteomic analyses, are needed to confirm these patterns and their biological significance.

Center of Assisted Reproduction Department of Obstetrics and Gynecology University Hospital and Masaryk University Brno Czechia

Deparment of Histology and Embryology Poznan University of Medical Sciences Poznan Poland

Department of Computer Science and Statistics Poznan University of Medical Sciences Poznan Poland

Department of Human Morphology and Embryology Division of Anatomy Faculty of Medicine Wrocław Medical University Wrocław Poland

Department of Immunology Poznan University of Medical Sciences Poznan Poland

Doctoral School Poznan University of Medical Sciences Poznan Poland

Greater Poland Center of Digital Medicine Poznan University of Medical Sciences Poznan Poland

Institute of Veterinary Medicine Nicolaus Copernicus University Torun Poland

Physiology Graduate Faculty North Carolina State University Raleigh NC United States

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