The recent popularity of indoor farming has brought about problems with parasites spreading among pollinator colonies. The natural product callunene (1) can be used in the prophylaxis of bumblebees against Crithidia infection. Here, we report the synthesis of callunene (1), its enantioseparation, and a method for analyzing its optical purity. The approach was applied to determine the configuration of callunene extracted from heather honey. The proposed method is also applicable to the analysis of mixtures of diastereomers obtained during callunene synthesis, which allows the stereospecificity of individual reaction steps to be determined.

Department of Analytical Chemistry Faculty of Chemical Engineering University of Chemistry and Technology Prague Technická 5 Prague 6 16628 Czech Republic

Department of Organic Chemistry Faculty of Chemical Technology University of Chemistry and Technology Prague Technická 5 Prague 6 16628 Czech Republic

Institute of Organic Chemistry and Biochemistry Czech Academy of Sciences Flemingovo nám 2 Prague 6 16000 Czech Republic

Laboratory of Nuclear Magnetic Resonance Spectroscopy University of Chemistry and Technology Prague Technická 5 Prague 6 16628 Czech Republic

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