OBJECTIVES: Given the high worldwide prevalence of vitamin D deficiency and its role in numerous diseases affecting mortality and morbidity, this study seeks to determine the prevalence of low 25-hydroxyvitamin D levels in the Czech Republic, where population-level data are currently lacking. STUDY DESIGN: This retrospective study utilized a large dataset to analyze 25-hydroxyvitamin D levels over an extended period. METHODS: We analyzed data from 119,925 individuals aged 0-100 years categorizing them as sufficient (75-250 nmol/L), insufficient (50-75 nmol/L), or deficient (<50 nmol/L). We also examined levels of CRP, homocysteine, and their correlations with 25-hydroxyvitamin D levels across age groups. Age, sex, sampling month, sunlight exposure (monthly and annual), and influenza virus positivity were assessed for their relationship with the 25-hydroxyvitamin D levels. RESULTS: The study found a high prevalence of 25-hydroxyvitamin D inadequacy, with sufficient levels observed in 65.6% of infants (0-12 months). The lowest prevalence of sufficiency was in the 6-15 years (19.2%) and 16-30 years (22.1%) groups. The highest deficiency prevalence was in the 91-100 years group (51.8%). 25-hydroxyvitamin D levels in all age groups and both sexes correlated with all selected parameters. Lower sun exposure, higher flu virus positivity, male gender, and elevated homocysteine and CRP levels were negatively correlated with 25-hydroxyvitamin D levels. CONCLUSIONS: The prevalence of 25-hydroxyvitamin D inadequacy in the Czech Republic is high. It is advisable to consider testing, monitoring, and providing medical recommendations for vitamin D supplementation as part of health prevention strategies in the general population.

Department of Clinical Immunology and Allergology University Hospital Hradec Kralove and Faculty of Medicine in Hradec Kralove Charles University 500 03 Hradec Kralove Czech Republic

Department of Medical Biophysics Faculty of Medicine in Hradec Kralove Charles University 500 03 Hradec Kralove Czech Republic

Department of Preventive Medicine Faculty of Medicine in Hradec Kralove Charles University 500 03 Hradec Kralove Czech Republic

Synlab czech s r o 186 00 Praha Czech Republic

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Melguizo-Rodríguez L, Costela-Ruiz VJ, García-Recio E, De Luna-Bertos E, Ruiz C, Illescas-Montes R. Role of Vitamin D in the Metabolic Syndrome. Nutrients. 2021;13:830. PubMed DOI PMC

Kheiri B, Abdalla A, Osman M, Ahmed S, Hassan M, Bachuwa G. Vitamin D deficiency and risk of cardiovascular diseases: a narrative review. Clin Hypertens. 2018;24:9.

Mazur A, Frączek P, Tabarkiewicz J. Vitamin D as a Nutri-Epigenetic Factor in Autoimmunity—A Review of Current Research and Reports on Vitamin D Deficiency in Autoimmune Diseases. Nutrients. 2022;14:4286. PubMed DOI PMC

Chandler PD, Chen WY, Ajala ON, Hazra A, Cook N, Bubes V, et al. Effect of Vitamin D3 Supplements on Development of Advanced Cancer: A Secondary Analysis of the VITAL Randomized Clinical Trial. JAMA Netw Open. 2020;3:e2025850–e. PubMed DOI PMC

Gröber U, Kisters K. Influence of drugs on vitamin D and calcium metabolism. Derm Endocrinol. 2012;4:158–66. DOI

Abboud M, Rybchyn MS, Rizk R, Fraser DR, Mason RS. Sunlight exposure is just one of the factors which influence vitamin D status. Photochemical Photobiological Sci. 2017;16:302–13. DOI

Margulies SL, Kurian D, Elliott MS, Han Z. Vitamin D deficiency in patients with intestinal malabsorption syndromes – think in and outside the gut. J Digestive Dis. 2015;16:617–33. DOI

Octavius GS, Shakila A, Meliani M, Halim A. Vitamin D deficiency is a public health emergency among Indonesian children and adolescents: a systematic review and meta-analysis of prevalence. Ann Pediatr Endocrinol Metab. 2023;28:10–9. PubMed DOI PMC

Cui A, Zhang T, Xiao P, Fan Z, Wang H, Zhuang Y. Global and regional prevalence of vitamin D deficiency in population-based studies from 2000 to 2022: A pooled analysis of 7.9 million participants. Front Nutr. 2023;10:1070808. PubMed DOI PMC

Holick MF, Binkley NC, Bischoff-Ferrari HA, Gordon CM, Hanley DA, Heaney RP, et al. Evaluation, Treatment, and Prevention of Vitamin D Deficiency: an Endocrine Society Clinical Practice Guideline. J Clin Endocrinol Metab. 2011;96:1911–30. PubMed DOI

Cui A, Xiao P, Ma Y, Fan Z, Zhou F, Zheng J, et al. Prevalence, trend, and predictor analyses of vitamin D deficiency in the US population, 2001–2018. Front Nutr. 2022;9:965376.

Mendes MM, Gomes APO, Araújo MM, Coelho ASG, Carvalho KMB, Botelho PB. Prevalence of vitamin D deficiency in South America: a systematic review and meta-analysis. Nutr Rev. 2023;81:1290–309. PubMed DOI

Xyda SE, Kotsa K, Doumas A, Papanastasiou E, Garyfallos AA, Samoutis G. Could the Majority of the Greek and Cypriot Population Be Vitamin D Deficient? Nutrients. 2022;14:3778. PubMed DOI PMC

Manios Y, Moschonis G, Lambrinou C-P, Tsoutsoulopoulou K, Binou P, Karachaliou A, et al. A systematic review of vitamin D status in southern European countries. Eur J Nutr. 2018;57:2001–36. PubMed DOI

Brouwer-Brolsma EM, Vaes AMM, van der Zwaluw NL, van Wijngaarden JP, Swart KMA, Ham AC, et al. Relative importance of summer sun exposure, vitamin D intake, and genes to vitamin D status in Dutch older adults: The B-PROOF study. J Steroid Biochem Mol Biol. 2016;164:168–76. PubMed DOI

Rabenberg M, Scheidt-Nave C, Busch MA, Rieckmann N, Hintzpeter B, Mensink GB. Vitamin D status among adults in Germany-results from the German Health Interview and Examination Survey for Adults (DEGS1). BMC Public Health. 2015;15:641. PubMed DOI PMC

Shatylo S, Bogomaz V, Babych O. Vitamin D deficiency in Ukraine: A multicentre cross-sectional study. Glob Epidemiol. 2024;8:100170. PubMed DOI PMC

Bronský J, Kalvachová B, Kutílek Š, Šebková A, Škvor J, Šumník Z, et al. Doporučený postup České pediatrické společnosti a Odborné společnosti praktických dětských léikaů ČLS JEP pro suplementaci dětí a dospívajících vitaminem D. 2019.

Uday S, Kongjonaj A, Aguiar M, Tulchinsky T, Högler W. Variations in infant and childhood vitamin D supplementation programmes across Europe and factors influencing adherence. Endocr Connect. 2017;6:667–75. PubMed DOI PMC

Kasemsripitak S, Jaruratanasirikul S, Boonrusmee S, Saengkaew T, Sriplung H. Prevalence and risk factors for vitamin D insufficiency in 6-12-month-old infants: a cross-sectional study in Southern Thailand. BMC Pediatr. 2022;22:729. PubMed DOI PMC

Choi YJ, Kim MK, Jeong SJ. Vitamin D deficiency in infants aged 1 to 6 months. Korean J Pediatr. 2013;56:205–10. PubMed DOI PMC

Zhang H, Li Z, Wei Y, Fu J, Feng Y, Chen D, et al. Status and influential factors of vitamin D among children aged 0 to 6 years in a Chinese population. BMC Public Health. 2020;20:429. PubMed DOI PMC

ní Chaoimh C, McCarthy EK, Hourihane JOB, Kenny LC, Irvine AD, Murray DM, et al. Low vitamin D deficiency in Irish toddlers despite northerly latitude and a high prevalence of inadequate intakes. Eur J Nutr. 2018;57:783–94. PubMed DOI

Stounbjerg NG, Mølgaard C, Cashman KD, Michaelsen KF, Damsgaard CT. Vitamin D status of 3-year-old children in Denmark: determinants and associations with bone mineralisation and blood lipids. Eur J Nutr. 2023;62:1441–51. PubMed DOI

Melissa S-B, Marco V-S, Ariel B-G, Ileana H-S. Prevalence of vitamin D deficiency in Costa Rican children. Acta Méd Costarric. 2021;60:104–13.

Drali O, Arab M, Lamdjadani N, Guechi Z, Berrah H. Vitamin D status in preschool children in Algeria. Arch De Pédiatrie. 2021;28:215–21. DOI

Horton-French K, Dunlop E, Lucas RM, Pereira G, Black LJ. Prevalence and predictors of vitamin D deficiency in a nationally representative sample of Australian adolescents and young adults. Eur J Clin Nutr. 2021;75:1627–36. PubMed DOI PMC

Hu Y, Jiang S, Lu J, Yang Z, Yang X, Yang L. Vitamin D Status for Chinese Children and Adolescents in CNNHS 2016-2017. Nutrients. 2022; 14:4928.

Galeazzi T, Quattrini S, Pjetraj D, Gatti S, Monachesi C, Franceschini E, et al. Vitamin D status in healthy Italian school-age children: a single-center cross-sectional study. Ital J Pediatrics. 2023;49:27. DOI

Sochorová L, Hanzlíková L, Černá M, Vosátková M, Grafnetterová AP, Fialová A, et al. Assessment of vitamin D status in Czech children. Cent Eur J Public Health. 2018;26:260–4. PubMed DOI

Rodgers JL, Jones J, Bolleddu SI, Vanthenapalli S, Rodgers LE, Shah K, et al. Cardiovascular Risks Associated with Gender and Aging. J Cardiovasc Dev Dis. 2019;6:19.

Marion CD, Stewart C, David S. Influence of age on the prevalence and components of the metabolic syndrome and the association with cardiovascular disease. BMJ Open Diab Res Care. 2016;4:e000195. DOI

White MC, Holman DM, Boehm JE, Peipins LA, Grossman M, Henley SJ. Age and cancer risk: a potentially modifiable relationship. Am J Prev Med. 2014;46:S7–15. PubMed DOI PMC

Kupisz-Urbańska M, Broczek K, Galus K, Mossakowska M, Marcinowska-Suchowierska E. Age-related differences in vitamin D status in Polish centenarians compared with 65-year-olds. Pol Arch Intern Med. 2020;130:853–9. PubMed

Foroni MZ, Cendoroglo MS, Sakane EN, Marin-Mio RV, Moreira P, Maeda SS, et al. Serum 25 hydroxyvitamin D concentrations in individuals over 80 years old and their correlations with musculoskeletal and health parameters. Endocrine. 2023;79:559–70. PubMed DOI

Rabenberg M, Scheidt-Nave C, Busch MA, Rieckmann N, Hintzpeter B, Mensink GBM. Vitamin D status among adults in Germany – results from the German Health Interview and Examination Survey for Adults (DEGS1). BMC Public Health. 2015;15:641. PubMed DOI PMC

Conzade R, Koenig W, Heier M, Schneider A, Grill E, Peters A, et al. Prevalence and Predictors of Subclinical Micronutrient Deficiency in German Older Adults: Results from the Population-Based KORA-Age Study. Nutrients. 2017;9:1276.

Elliott TM, Gordon LG, Webb A, Kift R, Foeglein A, Neale RE. Making the sunshine vitamin – How much sun exposure is needed to maintain 25-hydroxy vitamin D concentration? Photochemistry Photobiol. 2024;100:746–55. DOI

Fayet-Moore F, Brock KE, Wright J, Ridges L, Small P, Seibel MJ, et al. Determinants of vitamin D status of healthy office workers in Sydney, Australia. J Steroid Biochem Mol Biol. 2019;189:127–34. PubMed DOI

Osredkar J, Vičič V, Hribar M, Benedik E, Siuka D, Jerin A, et al. Seasonal variation of total and bioavailable 25-hydroxyvitamin D [25(OH)D] in the healthy adult Slovenian population. Acta Biochim Pol. 2024;71:13108. PubMed DOI PMC

Wierzbicka A, Oczkowicz M. Sex differences in vitamin D metabolism, serum levels and action. Br J Nutr. 2022;128:2115–30. PubMed DOI

Zhu Z, Zhu X, Gu L, Zhan Y, Chen L, Li X. Association Between Vitamin D and Influenza: Meta-Analysis and Systematic Review of Randomized Controlled Trials. Front Nutr. 2021;8:799709. PubMed DOI

Martineau AR, Jolliffe DA, Hooper RL, Greenberg L, Aloia JF, Bergman P, et al. Vitamin D supplementation to prevent acute respiratory tract infections: systematic review and meta-analysis of individual participant data. BMJ. 2017;356:i6583. PubMed DOI PMC

Smith AD, Refsum H. Homocysteine – from disease biomarker to disease prevention. J Intern Med. 2021;290:826–54. PubMed DOI

Peikert A, Kaier K, Merz J, Manhart L, Schäfer I, Hilgendorf I, et al. Residual inflammatory risk in coronary heart disease: incidence of elevated high-sensitive CRP in a real-world cohort. Clin Res Cardiol. 2020;109:315–23. PubMed DOI

Zhou A, Hyppönen E. Vitamin D deficiency and C-reactive protein: a bidirectional Mendelian randomization study. Int J Epidemiol. 2023;52:260–71. PubMed DOI

Laird E, O’Halloran AM, Molloy AM, Healy M, Bourke N, Kenny RA. Vitamin D status & associations with inflammation in older adults. PLoS One. 2023;18:e0287169. PubMed DOI PMC

Verdoia M, Nardin M, Gioscia R, Saghir Afifeh AM, Viglione F, Negro F, et al. Association between vitamin D deficiency and serum Homocysteine levels and its relationship with coronary artery disease. J Thrombosis Thrombolysis. 2021;52:523–31. DOI

Amer M, Qayyum R. The Relationship Between 25-Hydroxyvitamin D and Homocysteine in Asymptomatic Adults. J Clin Endocrinol Metab. 2014;99:633–8. PubMed DOI