This study investigates a synergistic combination of hollow fiber-based liquid phase microextraction (HF-LPME) and voltammetric analysis using screen-printed boron-doped diamond electrodes (SP-BDDE) for the determination of antihypertensive drug lercanidipine (LCN) in urine, blood plasma, and blood serum. Optimum HF-LPME conditions were: dodecane as supported liquid membrane, Britton-Robinson buffer (BRB) pH 7 as donor solution, BRB pH 3 as acceptor solution, and extraction time 30 min. Square-wave voltammetry (SWV) of 10 μL acceptor solution at SP-BDDE was utilized with amplitude 75 mV, frequency 10 Hz, and potential step 4 mV. The calibration dependence of the combined HF-LPME-SWV method was established in the range of 1-1000 nmol L-1, with limits of quantification (LOQ) and detection (LOD) of 3.3 and 1.1 nmol L-1, respectively. The applicability of the developed method was verified by analysis of human urine, blood plasma, and blood serum samples spiked with 20 and 100 nmol L-1 LCN with recoveries ranging from 93.2 to 112.9 % with respective RSDs 1.72-8.5 % (n = 3).

J Heyrovsky Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic Dolejškova 3 182 00 Prague 8 Czech Republic

J Heyrovsky Institute of Physical Chemistry of the Academy of Sciences of the Czech Republic Dolejškova 3 182 00 Prague 8 Czech Republic; University of Pardubice Faculty of Chemical Technology Institute of Environmental and Chemical Engineering Studentská 573 532 10 Pardubice Czech Republic

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