Minimal residual disease assessment following CD19-targeted therapy in B-cell precursor acute lymphoblastic leukemia using standardized 12-color flow cytometry: A EuroFlow study
Status PubMed-not-MEDLINE Language English Country United States Media electronic-ecollection
Document type Journal Article
PubMed
40224162
PubMed Central
PMC11993931
DOI
10.1002/hem3.70125
PII: HEM370125
Knihovny.cz E-resources
- Publication type
- Journal Article MeSH
Detection of minimal/measurable residual disease (MRD) is a critical prognostic marker in B-cell precursor acute lymphoblastic leukemia (BCP-ALL). The EuroFlow Consortium previously developed an 8-color flow cytometric MRD protocol, effective for >98% of BCP-ALL patients treated with chemotherapy. This study aimed to enhance MRD detection, particularly for patients treated with CD19-targeted therapies, by expanding the EuroFlow protocol to a 12-color panel. This new panel incorporates additional B-cell markers and exclusion T/NK-cell markers (CD3 and CD7). Through an evaluation of 237 diagnostic BCP-ALL samples, CD22, CD24, and HLA-DR were selected as additional B-cell gating markers. Two 12-color tubes were technically optimized and clinically validated across 101 patient follow-up samples, demonstrating excellent concordance with molecular MRD levels (R 2 = 0.88). The 12-color BCP-ALL MRD tubes were compatible with the previously developed 8-color automated gating and identification (AGI) tool and demonstrated good reproducibility. Our findings indicate that the 12-color panel performs comparably to the 8-color BCP-ALL MRD panel, including both CD19-positive and CD19-negative cases. However, it offers an improved definition of the B-cell lineage, particularly for expert-guided manual data analysis, and provides additional information on the expression of the targetable marker CD22.
Clinic 3 Special Hematology Laboratory Rostock University Medical Center Rostock Germany
Department of Diagnostic Sciences Ghent University Ghent Belgium
Department of Hematology University of Schleswig Holstein Campus Kiel Kiel Germany
Department of Immunology LUMC Leiden The Netherlands
Department of Laboratory Medicine Ghent University Hospital Ghent Belgium
Department of Microbiology and Immunology Medical University of Silesia Katowice Poland
Department of Pediatric Hematology and Oncology Medical University of Silesia Katowice Poland
Institute of Biomedical Research of Salamanca Salamanca Spain
Instituto Português de Oncologia de Lisboa Francisco Gentil Rua Prof Lima Basto Lisboa Portugal
Pediatrics Fondazione IRCCS San Gerardo dei Tintori Monza Italy
Princess Máxima Center for Pediatric Oncology Utrecht The Netherlands
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