Stilbenes are plant secondary metabolites with remarkable antidiabetic, anti-inflammatory, antimicrobial, antioxidant, antitumor, and neuroprotective properties. As these compounds are valuable constituents in healthcare products and promising drug candidates, exploring new sources of stilbenes is essential for therapeutic advancement. The present study reports the isolation of two stilbene glycosides, resveratroloside and pinostilbenoside, from Pinus cembra L. bark. Their antioxidant activity and cytotoxic effects against HeLa cells were evaluated in comparison to the raw bark extract. The structures of resveratroloside and pinostilbenoside were confirmed by nuclear magnetic resonance (NMR) and mass spectrometry (MS) data analyses. Antioxidant activity was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and reducing power assays. Cell viability, apoptosis, cell proliferation, and cell cycle assays were used to evaluate the cytotoxic potential against HeLa cells. Resveratroloside and pinostilbenoside exhibited lower activity as free radical scavengers and reducing agents. However, they showed greater efficacy in reducing viability and suppressing proliferation in human cervical carcinoma HeLa cells. Given the promising findings of our study, the therapeutic potential of resveratroloside and pinostilbenoside should be further investigated.

Centre for Natural Products Discovery School of Pharmacy and Biomolecular Sciences Liverpool John Moores University Byrom Street Liverpool L3 3AF UK

Faculty of Chemical Engineering and Environmental Protection Gheorghe Asachi Technical University of Iasi 73 Prof Dr Docent Dimitrie Mangeron Street 700050 Iasi Romania

Faculty of Chemistry Alexandru Ioan Cuza University of Iasi 11 Carol 1 Boulevard 700506 Iasi Romania

Faculty of Medicine Grigore T Popa University of Medicine and Pharmacy 16 Universitatii Street 700115 Iasi Romania

Faculty of Pharmacy Grigore T Popa University of Medicine and Pharmacy 16 Universitatii Street 700115 Iasi Romania

Institute of Biological Research Iasi Branch of NIRDBS National Institute of Research and Development for Biological Sciences 47 Lascar Catargi Street 700107 Iasi Romania

Institute of Interdisciplinary Research CERNESIM Centre Alexandru Ioan Cuza University of Iasi 11 Carol 1 Boulevard 700506 Iasi Romania

Laboratory of Growth Regulators Palacký University and Institute of Experimental Botany The Czech Academy of Sciences Šlechtitelů 27 78371 Olomouc Czech Republic

Medical Investigations Praxis SRL 35 Moara de Vant Street 700376 Iasi Romania

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