Helicases and endonucleases play crucial roles in genome maintenance by unwinding or cleaving various forms of DNA and RNA structures in order to facilitate essential biological processes, such as DNA replication and recombination. Here, we identified fission yeast Dbl2 as a potential interactor of several complexes that exhibit either helicase or endonuclease activity, namely Fml1-MHF, SCFFbh1, Rqh1-Top3-Rmi1, and Mus81-Eme1. In vitro, Dbl2 binds to DNA, with a preference for branched molecules, such as D-loops, mobile Holliday junctions, and fork structures, making it a good candidate to play a central role in modulating the activity of helicases and endonucleases during replication and recombination repair. Previously, we showed that Dbl2 recruits Fbh1 to the ongoing homologous recombination sites, affecting the Rad51-nucleofilament. In this study, we determined that deleting dbl2 in an fbh1Δ background did not increase sensitivity to DNA-damaging agents or the frequency of Tf2 ectopic recombination. Therefore, Dbl2 and Fbh1 might be involved in the same molecular pathway, maintaining genome integrity by hindering ectopic recombination at repetitive elements.

Biomedical Research Center Department of Genetics Slovak Academy of Sciences Dubravska Cesta 9 845 05 Bratislava Slovakia

Department of Biology Faculty of Medicine Masaryk University Kamenice 5 Brno 625 00 Czechia

Department of Cell Biology Faculty of Science Charles University 128 00 Praha 2 Czechia

Department of Chromosome Biology University of Vienna Vienna Biocenter Dr Bohr Gasse 9 1030 Vienna Austria

Department of Genetics Faculty of Natural Sciences Comenius University in Bratislava 841 04 Bratislava Slovakia

Department of Molecular Biotechnology and Microbiology Faculty of Science and Technology University of Debrecen 4010 Debrecen Hungary

Faculty of Science National Centre for Biomolecular Research Masaryk University Kamenice 5 625 00 Brno Czechia

Institute of Animal Biochemistry and Genetics Centre of Biosciences Slovak Academy of Sciences 840 05 Bratislava Slovakia

Institute of Biochemistry and Biophysics Polish Academy of Sciences 02 106 Warsaw Poland

Institute of Chemistry Slovak Academy of Sciences Dubravska Cesta 9 845 38 Bratislava Slovakia

Institute of Microbial Genetics Department of Agricultural Sciences BOKU University Vienna Campus Tulln Konrad Lorenz Strasse 24 3430 Tulln an der Donau Austria

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