Collagen membranes are widely used in tissue and bone engineering, including guided bone regeneration (GBR). For effective and uninterrupted bone healing, a GBR membrane must maintain its functionality for an initial critical period of 4 weeks. A novel carp collagen sponge has already shown promise as a wound coating and vascular graft coating, making it a candidate for GBR applications as well. To enhance the mechanical properties and longevity of GBR membranes, we modified the basic carp collagen membrane with combinations of l-lactide, ε-caprolactone, d,l-lactide, and glycolide in various molar ratios. While traditional methods rely on histological evaluation to assess the degradation pattern and therefore suitability of GBR membranes ex vivo, this study employed micro-MRI as an innovative, noninvasive approach to monitor the in vivo degradation of carp collagen membrane and its polymer-modified variants. Our findings demonstrated that micro-MRI is a reliable and effective method for visualizing collagen membrane degradation in vivo, up to scaffold disintegration. Among the variants tested, collagen GBR membrane coated with d,l-lactide and glycolide in a 50:50 M ratio emerged as the most suitable for GBR purposes. However, since this study was conducted in the subcutaneous tissue of a rat model, further research is required to determine the behavior of carp collagen GBR membrane variants on bony surfaces.

1st Faculty of Medicine Charles University Prague Czech Republic

Center for Advanced Preclinical Imaging 1st Faculty of Medicine Charles University Prague Czech Republic

Department of Composites and Carbon Materials Institute of Rock Structure and Mechanics Czech Academy of Sciences Prague Czech Republic

Department of Oral and Maxillofacial Surgery General University Hospital Prague Prague Czech Republic

Faculty of Medicine in Pilsen Charles University Pilsen Czech Republic

Institute of Pathology Charles University Prague Czech Republic

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