In the course of biochemical prospection of Laburnicola nematophila isolated from eggs of the plant-parasitic cyst nematode Heterodera filipjevi, eight 2-pyridone alkaloids were isolated from its solid-state BRFT cultures and identified as six previously undescribed, cordypyridones E-J (1-6), and two known congeners, cordypyridones C (7) and D (8). The structures and absolute configurations of all isolated compounds were elucidated through HR-ESI-MS, 1D/2D NMR spectroscopy, and TDDFT-ECD calculations. Compound 4, bearing a rare N-hydroxy-2-pyridone moiety structurally related to that of PF1140, exhibited broad-spectrum bioactivity in cytotoxicity and antimicrobial assays. Compounds 5 and 6 demonstrated potent antibiofilm activities against Staphylococcus aureus, reducing its biofilm formation by almost 50% at 0.25 and 7.8 μg/mL, respectively.

Biotechnology Research Center and Department of Biotechnology Toyama Prefectural University 5180 Kurokawa Imizu Toyama 939 0398 Japan

Computational Chemistry Laboratory Chemistry Department Faculty of Science Minia University Minia 61519 Egypt

Department of Engineering College of Engineering and Technology University of Technology and Applied Sciences Nizwa 611 Sultanate of Oman

Department of Microbial Drugs Helmholtz Centre for Infection Research GmbH Inhoffenstraße 7 38124 Braunschweig Germany

Department of Pharmacognosy Faculty of Pharmacy Ain Shams University 11566 Cairo Egypt

Department of Zoology and Entomology University of the Free State Bloemfontein 9300 South Africa

Institute for Crop and Soil Science Julius Kühn Institute Federal Research Centre for Cultivated Plants Bundesallee 58 38116 Braunschweig Germany

Institute for Epidemiology and Pathogen Diagnostics Julius Kühn Institute Federal Research Centre for Cultivated Plants Messeweg 11 12 38104 Braunschweig Germany

Institute of Microbiology Technische Universität Braunschweig Spielmannstraße 7 38106 Braunschweig Germany

Laboratory of Fungal Genetics and Metabolism Institute of Microbiology of the Czech Academy of Sciences 14220 Prague Czechia

School of Health Sciences University of KwaZulu Natal Westville Campus Durban 4000 South Africa

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