A chemical investigation of Laburnicola nematophila, isolated from cysts of the plant parasitic nematode Heterodera filipjevi, affored three dactylfungin derivatives (1-3) and three tetralone congeners (4-6). Dactylfungin C (1), laburnicolin (4), and laburnicolenone (5) are previously undescribed natural products. Chemical structures of the isolated compounds were determined based on 1D and 2D NMR spectroscopic analyses together with HR-ESI-MS spectrometry and comparison with data reported in the literature. The relative configurations of compounds 1, 2, and 4-6 were determined based on their ROESY data and analysis of their coupling constants (J values). The absolute configurations of 4-6 were determined through the comparison of their measured and calculated TDDFT-ECD spectra. Compounds 1-3 were active against azole-resistant Aspergillus fumigatus.

Department of Microbial Drugs Helmholtz Centre for Infection Research and German Centre for Infection Research Inhoffenstrasse 7 38124 Braunschweig Germany

Department of Organic Chemistry University of Debrecen P O Box 400 4002 Debrecen Hungary

Department of Pharmacognosy Faculty of Pharmacy Ain Shams University Cairo 11566 Egypt

Institute for Crop and Soil Science Julius Kühn Institute Federal Research Centre for Cultivated Plants Bundesallee 58 38116 Braunschweig Germany

Institute for Epidemiology and Pathogen Diagonstics Julius Kühn Institut Federal Research Centre for Cultivated Plants Messeweg 11 12 38104 Braunschweig Germany

Institute of Microbiology Czech Academy of Science Vídeňská 1083 14220 Prague Czech Republic

Institute of Microbiology Technische Universität Braunschweig Spielmannstraße 7 38106 Braunschweig Germany

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