A microfluidic reverse transcription loop-mediated isothermal amplification (RT-LAMP) platform is presented featuring integrated real-time fluorescence detection and optimized thermal control. Magnetic bead-based RNA extraction was paired with a thermally insulated reaction chip operated under vacuum to suppress heat loss. Additional energy-saving strategies, including reduced wire cross-section and low-emissivity surface treatment, reduced total power consumption from (2.00 ± 0.08) W to (0.29 ± 0.01) W, corresponding to an 85.5 % reduction. Amplification was completed within 20 min, and positive fluorescence signals were detected in less than 11 min. Although LAMP reagents remain more expensive than those used in PCR, the substantial energy savings and system simplification demonstrated here support practical battery-powered operation. The RT-LAMP chemistry used in this system matched a previously validated configuration, which achieved a limit of detection of 256 RNA copies and a signal slope of (- 1.70 ± 0.20) dec.min⁻1. These parameters remained consistent under the current setup, as power consumption did not influence detection sensitivity provided the reaction temperature was maintained. The platform enables low-power, real-time nucleic acid detection suited for decentralized diagnostics, forensic screening, and environmental monitoring.

Currently at Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 PR China

Department of Chemistry and Biochemistry Mendel University in Brno Zemědělská 1 Brno 61300 Czech Republic

Department of Microelectronics Faculty of Electrical Engineering and Communication Brno University of Technology Technická 3058 10 Brno 61600 Czech Republic

Ministry of Education Key Laboratory of Micro and Nano Systems for Aerospace School of Mechanical Engineering Northwestern Polytechnical University 127 West Youyi Road Xi'an Shaanxi 710072 PR China

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