During infectious disease outbreaks, the centers for disease control need to monitor particular areas. Considerable effort has been invested in the development of portable, user-friendly, and cost-effective systems for point-of-care (POC) diagnostics, which could also create an Internet of Things (IoT) for healthcare via a global network. However, at present IoT based on a functional POC instrument is not available. Here we show a fast, user-friendly, and affordable IoT system based on a miniaturized polymerase chain reaction device. We demonstrated the system's capability by amplification of complementary deoxyribonucleic acid (cDNA) of the dengue fever virus. The resulting data were then automatically uploaded via a Bluetooth interface to an Android-based smartphone and then wirelessly sent to a global network, instantly making the test results available anywhere in the world. The IoT system presented here could become an essential tool for healthcare centers to tackle infectious disease outbreaks identified either by DNA or ribonucleic acid.

Air Force Military Medical University 169 Changle West Road Xi'an Shaanxi 710032 PR China

Brno University of Technology Central European Institute of Technology Purkyňova 123 61200 Brno Czech Republic

Czech Technical University Prague Technická 2 166 27 Praha 6 Czech Republic

Institute for Infocomm Research ASTAR 1 Fusionopolis Way 21 01 Connexis 138632 Singapore

Ministry of Education Key Laboratory of Micro Nano Systems for Aerospace School of Mechanical Engineering Northwestern Polytechnical University Xi'an 710072 PR China

National Institute for Research and Development in Microtechnologies IMT Bucharest Bucharest 077190 Romania

School of Life Science Northwesstern Polytechnical University Xi'an 710072 PR China

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