We show the utilization of a recently developed cellphone-sized real-time polymerase chain reaction (PCR) device to detect Ebola virus RNA using single-step reverse transcription PCR (RT-PCR). The device was shown to concurrently perform four PCRs, each with a sample volume of 100 nL: one positive control with both Ebola and GAPDH RNA and one negative control. The last two positions were used to measure the GAPDH and the Ebola content of a sample. A comparison of threshold cycles (CT) from the two samples provided relative quantification. The entire process, which consisted of reverse transcription, PCR amplification, and melting curve analysis (MCA), was conducted in less than 37 min. The next step will be integration with a sample preparation unit to form an integrated sample-to-answer system for point-of-care infectious disease diagnostics.

Brno University of Technology Central European Institute of Technology Antonínská 548 1 CZ 601 90 Brno Czech Republic

KIST Europe Campus E7 1 66123 Saarbrücken Germany

Northwestern Polytechnical University School of Mechanical Engineering Department of Microsystem Engineering 127 West Youyi Road Xi'an Shaanxi 710072 P R China

Citace poskytuje Crossref.org

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