Inter-laboratory trials were carried out to evaluate the reproducibility and estimate test performance characteristics of the Phagomagnetic separation (PhMS)-qPCR assay, a novel phage-based assay recently developed as a rapid alternative to culture for detecting viable Mycobacterium avium subsp. paratuberculosis (MAP) in bovine milk and feces. Unique reagents and a detailed instruction manual required for the PhMS-qPCR assay were provided to five European veterinary diagnostic laboratories by Rapid-Myco Technologies Limited. Milk and feces test panels were prepared at NEIKER and distributed to participant laboratories between April-June 2023 and March-May 2024, respectively. Each test panel comprised of MAP-spiked and/or naturally infected bovine milk or feces samples (18 samples per panel on two separate occasions for each sample matrix). The six participant laboratories (including organizer) performed automated or manual PhMS and used whatever qPCR instrument they had available. All laboratories used the IDEXX RealPCR MAP DNA test for the qPCR part of the assay. Generally, substantial agreement was observed overall between PhMS-qPCR results and reference culture results for spiked milk (Kappa value 0.5982) and naturally MAP-infected feces (Kappa value 0.7780 using an amended protocol). Preliminary estimates of the detection (analytical) sensitivity (Se), detection specificity (Sp) and trueness (T) of the PhMS-qPCR assay applied to bovine milk and feces were obtained. The mean Se, Sp, and T values across six laboratories were 93.1%, 67.9%, and 88.7% when milk was tested and 84.1%, 93.7%, and 88.9% when feces was tested. Overall, the PhMS-qPCR assay performed well in multiple laboratories and test reproducibility was demonstrated (Cohen's Kappa ≥0.6-1.000). The estimates of performance characteristics of the PhMS-qPCR assay are generally acceptable for a potential diagnostic test. Hence the PhMS-qPCR assay shows considerable promise as a rapid test to detect viable MAP in veterinary specimens such as milk and feces. Further and fuller validation of the assay will continue to assess its diagnostic potential.

ANSES Ploufragan Plouzané Niort Laboratory Ruminant Diseases and Welfare Unit Niort France

Centro de Vigilancia Sanitaria Veterinaria Universidad Complutense de Madrid Madrid Spain

Departamento de Genética Fisiología y Microbiología Facultad de Ciencias Biológicas Universidad Complutense de Madrid Madrid Spain

Departamento de Sanidad Animal Facultad de Veterinaria Universidad Complutense de Madrid Madrid Spain

Departamento de Sanidad Animal NEIKER Instituto Vasco de Investigación y Desarrollo Agrario Basque Research and Technology Alliance Derio Bizkaia Spain

Department of Microbiology and Antimicrobial Resistance Veterinary Research Institute Brno Czechia

GDS France Paris France

Institute of Molecular Pathogenesis Friedrich Loeffler Institut Jena Germany

Rapid Myco Technologies Limited Belfast United Kingdom

School of Biological Sciences Queen's University Belfast Belfast United Kingdom

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