- Oždian, Tomáš
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Vodička, Jan
Autor Vodička, Jan Department of Gynecology and Obstetrics, University Hospital in Olomouc, Faculty of Medicine and Dentistry, Palacký University in Olomouc, Hněvotínská 3, 779 00 Olomouc, Czech Republic Department of Gynecology and Obstetrics, University Hospital in Olomouc, I.P.Pavlova 6, 779 00 Olomouc, Czech Republic
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Dostál, Jiří
Autor Dostál, Jiří ORCID Department of Gynecology and Obstetrics, University Hospital in Olomouc, Faculty of Medicine and Dentistry, Palacký University in Olomouc, Hněvotínská 3, 779 00 Olomouc, Czech Republic Department of Gynecology and Obstetrics, University Hospital in Olomouc, I.P.Pavlova 6, 779 00 Olomouc, Czech Republic
- Holub, Dušan
- Václavková, Jana
- Ješeta, Michal
- Hamerníková, Barbora
- Kouřilová, Pavla
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Malchar, Ondřej
Autor Malchar, Ondřej Department of Gynecology and Obstetrics, University Hospital in Olomouc, Faculty of Medicine and Dentistry, Palacký University in Olomouc, Hněvotínská 3, 779 00 Olomouc, Czech Republic Department of Gynecology and Obstetrics, University Hospital in Olomouc, I.P.Pavlova 6, 779 00 Olomouc, Czech Republic
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Dvořák, Vladimír
Autor Dvořák, Vladimír ORCID Department of Gynecology and Obstetrics, University Hospital in Olomouc, Faculty of Medicine and Dentistry, Palacký University in Olomouc, Hněvotínská 3, 779 00 Olomouc, Czech Republic Department of Gynecology and Obstetrics, University Hospital in Olomouc, I.P.Pavlova 6, 779 00 Olomouc, Czech Republic
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PubMed
36674559
DOI
10.3390/ijms24021038
Knihovny.cz E-zdroje
Cervical mucus (CM) is a viscous fluid that is produced by the cervical glands and functions as a uterine cervix plug. Its viscosity decreases during ovulation, providing a window for non-invasive sampling. This study focuses on proteomic characterization of CM to evaluate its potential as a non-invasively acquired source of biomarkers and in understanding of molecular (patho)physiology of the female genital tract. The first objective of this work was to optimize experimental workflow for CM processing and the second was to assess differences in the proteomic composition of CM during natural ovulatory cycles obtained from intrauterine insemination (IUI) cycles and in vitro fertilization (IVF) cycles with controlled ovarian hyperstimulation. Proteomic analysis of CM samples revealed 4370 proteins involved in processes including neutrophil degranulation, cellular stress responses, and hemostasis. Differential expression analysis revealed 199 proteins enriched in IUI samples and 422 enriched in IVF. The proteins enriched in IUI were involved in phosphatidic acid synthesis, responses to external stimulus, and neutrophil degranulation, while those enriched in IVF samples were linked to neutrophil degranulation, formation of a cornified envelope and hemostasis. Subsequent analyses clarified the protein composition of the CM and how it is altered by hormonal stimulation of the uterus.
- MeSH
- biologické markery MeSH
- cervikální hlen * MeSH
- fertilizace in vitro MeSH
- lidé MeSH
- proteom MeSH
- proteomika MeSH
- umělá inseminace * MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
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