This study was done to identify the content compounds of Achillea wilhelmsii (A. wilhelmsii) and to evaluate its hypoglycemic and anti-hypercholesterolemic activity and effect on inflammatory mediators. The extracts and fractions of A. wilhelmsii were thoroughly analyzed using high performance liquid chromatography (HPLC), and the total content of phenols and flavonoids was determined. The hypoglycemic activity was evaluated in vivo using alloxan-induced diabetic mice. The effect upon inflammatory mediators was evaluated in vitro using the human monocytic leukemia cell line (THP-1). The anti-hypercholesterolemic activity was evaluated in vitro using the 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase assay kit. The water extract (WE)-treated group showed the highest reduction in the fasting blood glucose levels (FBGL). The chloroform fraction (CF) and ethyl acetate fraction (EAF) both showed a significant ability to reduce the secretion of tumor necrosis factor alpha (TNF-α). The EAF, however, also attenuated the levels of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9). The CF showed the most significant 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) inhibition activity. The five main compounds in the CF were isolated and identified. Out of the five compounds in the CF, 1β,10β-epoxydesacetoxymatricarin (CP1) and leucodin (CP2) showed the highest anti-hypercholesterolemic potential. A molecular docking study provided corresponding results.
- MeSH
- acylkoenzym A chemie MeSH
- antioxidancia aplikace a dávkování chemie MeSH
- buněčné linie MeSH
- experimentální diabetes mellitus farmakoterapie MeSH
- fenoly aplikace a dávkování chemie izolace a purifikace MeSH
- flavonoidy aplikace a dávkování chemie izolace a purifikace MeSH
- hypercholesterolemie farmakoterapie MeSH
- hyperglykemie farmakoterapie MeSH
- lidé MeSH
- mediátory zánětu chemie MeSH
- myši inbrední NOD MeSH
- myši MeSH
- řebříček chemie MeSH
- rizikové faktory MeSH
- rostlinné extrakty aplikace a dávkování chemie MeSH
- simulace molekulového dockingu MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- zánět farmakoterapie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
