The effects of gemfibrozil (GFZ), an antihyperlipidemic agent, on the anionic transport of the human red blood cells (RBC) during the oxygenation-deoxygenation cycle were examined. Gemfibrozil clearly plays a role in the modulation of the anionic flux in erythrocytes; in fact it causes a strong increment of anions transport when the RBCs are in the high-oxygenation state (HOS). Such an effect is remarkably reduced in the lowoxygenation state (LOS). With the aim of identifying the dynamics of fibrate action, this effect has been investigated also in human ghost and chicken erythrocytes. These latter, in fact, are known to possess a B3 (anion transporter or Band 3) modified at the cytoplasmic domain (cdb3) which plays a significant role in the metabolic modulation of red blood cells. The results were analyzed taking into account the well-known interactions between fibrates and both conformational states of hemoglobin i.e. the T state (deoxy-conformation) and the R state (oxy-conformation). The effect of gemfibrozil on anionic influx appears to be due to a wide interaction involving a “multimeric” Hb-GFZ-cdb3 macromolecular complex.
- Klíčová slova
- Sulfate transport, Oxygenation - deoxygenation,
- MeSH
- dospělí MeSH
- erytrocytární membrána MeSH
- erytrocyty metabolismus účinky léků MeSH
- financování organizované MeSH
- gemfibrozil farmakologie MeSH
- hemoglobiny metabolismus MeSH
- hypolipidemika farmakologie MeSH
- indikátory a reagencie MeSH
- kalibrace MeSH
- kinetika MeSH
- konformace proteinů MeSH
- lidé středního věku MeSH
- lidé MeSH
- proteinfosfatasy metabolismus MeSH
- pufry MeSH
- sírany krev MeSH
- spotřeba kyslíku fyziologie účinky léků MeSH
- vanadáty farmakologie MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
