Ecto-5'-nucleotidases are glycosyl phosphatidylinositol (GPI)-linked membrane-bound glycoproteins that convert extracellular AMP to adenosine. They play important roles in the inflammatory response where they modulate levels of pro-inflammatory extracellular ATP and anti-inflammatory extracellular adenosine. They are found in the saliva of blood feeding insects and also have a role in male reproduction. Drosophila possesses five genes with eight alternative transcripts encoding proteins with sequence homology to mammalian ecto-5'-nucleotidases. Here we show that two of them - NT5E-1 (CG4827) and NT5E-2 (CG30104) - are GPI-linked proteins with ecto-5'-nucleotidase activity but that they can also be released from the GPI anchor and exhibit secreted 5'-nucleotidase activity in growth media. The third locus in the cluster, CG30103, most likely also encodes a GPI-anchored membrane-bound protein but without 5'-nucleotidase activity, possibly due to the numerous substitutions in the amino acid sequence. Together with NT5E-2, CG30103 is also expressed in the testis offering an interesting model to investigate ecto-5'-nucleotidase enzymatic and extra-enzymatic function in male reproduction. CG42249 locus encoding two alternative transcripts is sequentially similar to family of apyrases related to 5'-nucleotidases and we show here that together with CG5276 belonging to another family of calcium-activated nucleotidases function as apyrases converting extracellular ATP to ADP and AMP. The last locus, CG11883, encodes most likely a cytoplasmic/mitochondrial protein.
- MeSH
- 5'-nukleotidasa genetika metabolismus MeSH
- apyrasa genetika metabolismus MeSH
- buněčné linie MeSH
- Drosophila melanogaster enzymologie genetika MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- multigenová rodina MeSH
- proteiny Drosophily genetika metabolismus MeSH
- sekvence aminokyselin MeSH
- vývojová regulace genové exprese MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
