Mortality and morbidity of newborns with sepsis can be improved by early and accurate diagnosis and targeted therapy. To evaluate the early molecular events associated with inflammation and infection, we evaluated markers of endothelial activation and injury and circulating plasma miRNAs in preterm newborns with sepsis. The study group consisted of newborns with gestational age ≤ 32 weeks, with culture-positive early-onset neonatal sepsis (sepsis group, N = 8), and as a control group, we enrolled newborns without sepsis (control group, N = 12). Soluble markers of inflammation were measured using Luminex-based multiplex assay. Platelet-free plasma RNA was used to construct the library for miRNA sequencing analysis. Normalized counts were calculated and used to measure differential expression of individual detected miRNAs. We found a significant increase of interleukin 18 (IL-18) in the cord blood of the sepsis group (mean ± SEM, 104.7 ± 30.4 pg/ml vs 52.7 ± 5.6 pg/ml, P = 0.02). In peripheral blood of sepsis group patients, we found a significant increase of VEGF-A compared to controls (196.0 ± 70.5 pg/ml vs 59.6 ± 8.5 pg/ml, P = 0.02). In the cord blood plasma, eight miRNAs had significantly differential expression (P < 0.05), four miRNAs were up-regulated and four miRNAs down-regulated. In peripheral blood plasma, all nine miRNAs with significant differential expression were up-regulated. In conclusion, in early-onset neonatal sepsis, IL-18 and VEGF-A might be considered in diagnostic workup. Early-onset sepsis in preterm newborns is associated with significant changes in the circulating miRNA pattern.

• MeSH
• biologické markery metabolismus   MeSH
• interleukin-18 MeSH
• kojenec MeSH
• lidé MeSH
• mikro RNA * genetika   MeSH
• novorozenec MeSH
• novorozenecká sepse * diagnóza   MeSH
• sepse * diagnóza   genetika   MeSH
• vaskulární endoteliální růstový faktor A MeSH
• zánět MeSH
• Check Tag
• kojenec MeSH
• lidé MeSH
• novorozenec MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• dějiny 20. století MeSH
• dítě MeSH
• lidé MeSH
• nemocniční oddělení dějiny   MeSH
• neonatologie * dějiny   organizace a řízení   pracovní síly   přístrojové vybavení   MeSH
• novorozenec MeSH
• péče o dítě MeSH
• Check Tag
• dějiny 20. století MeSH
• dítě MeSH
• lidé MeSH
• novorozenec MeSH
• Publikační typ
• historické články MeSH

• O autorovi
• Masarykova nemocnice (Ústí nad Labem, Česko) Autorita

• Klíčová slova
• neonatologie,
• MeSH
• neonatologie MeSH
• novorozenec MeSH
• Check Tag
• novorozenec MeSH

• NLK Obory
• perinatologie a neonatologie
• pediatrie

• MeSH
• kongresy jako téma MeSH
• kvalita zdravotní péče trendy   MeSH
• neonatologie * metody   trendy   MeSH