Background: The goal of our research is to show the effects and impacts of hyperbaric oxygen therapy (HBOT) on acute model wounds in animal subjects. Methods: Three experimental groups were created using injured rabbits (N=36)-randomly divided into three groups (N=12 per group). One group was treated only with standard wound care management. Two groups were additionally treated with HBOT either once or twice a day. The wounds were surgical, uninfected, and in healthy animal test subjects. We compared the immunohistochemical and histological parameters in 4-, 7- and 10-day intervals.Results: The detection of epidermal leaf parameters, the number of microabscesses, the Histopathological Superficial Epithelium Healing Score, Connective Tissue Healing Score, Histopathological Acute Inflammation Score and Total Histopathological Wound Healing Score all showed significant changes between time intervals within the individual groups.Conclusion: The results did not show that HBOT had a significant effect on the healing process of uncomplicated acute wounds.

• Klíčová slova
• Hyperbaric oxygen, Wound healing, Animal models, Adjunctive treatment,
• MeSH
• biopsie MeSH
• hojení ran MeSH
• hyperbarická oxygenace * metody   veterinární   MeSH
• králíci zranění   MeSH
• Check Tag
• králíci zranění   MeSH

• MeSH
• hyperbarická oxygenace * MeSH
• kongresy jako téma MeSH
• lidé MeSH
• nemoci mozku MeSH
• poranění mozku MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• zprávy MeSH

BACKGROUND: Pneumothorax as a consequence of pulmonary barotrauma during explosive decompression military crew training in a hypobaric chamber is an extremely rare and sparsely diagnosed complication. Extensive bilateral tissue damage is even more unexpected. CASE PRESENTATION: A 26-year-old active duty Air Force pilot was performing an explosive decompression simulation from 8000 ft. (2438.4 m) to 25,000 ft. (7620 m) in a 1.5 s interval. The training was interrupted due to the pilot's apparent health complications. After transfer to the emergency department, a CT scan showed bilateral lung barotrauma with emphysema. CONCLUSIONS: The case report shows extensive emphysema and pneumothorax after a rapid decompression done for training purposes. It is a possible but rare complication. The cause remains unclear, with suspicion of a predisposed lung disease.

• MeSH
• barotrauma etiologie   MeSH
• dekomprese explozivní * MeSH
• dospělí MeSH
• lidé MeSH
• plicní emfyzém komplikace   MeSH
• pneumotorax komplikace   MeSH
• počítačová rentgenová tomografie MeSH
• poškození plic MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH

Background: Hyperbaric oxygen (HBO2) therapy can have a positive effect on wound healing, angiogenesis and blood flow. No prior study has described the effects of HBO2 therapy and gene expression of this process. The goal of our research was to show the effects of HBO2 and its impact at the molecular level on angiogenesis, proliferation, differentiation, oxidative stress, inflammation, and extracellular matrix formation. Live animal subjects were used for simulating the process of wound healing under standard conditions and under the influence of HBO2. Methods: Two experimental groups were created using injured rabbits (N=24), one group (N=12) treated with hyperbaric therapy twice a day and one (N=12) with standard wound care management. Wounds were surgical, uninfected, and in healthy animal test subjects. We compared the whole genomic analysis of the transcriptome with the use of microarray technology at three intervals during treatment. Results: The induction of the wounds in rabbit skin increased expression of hundreds of genes in both treatment groups. The numbers of elevated and decreased genes gradually reduced as the wound healed. Gene expression analysis showed elevated expression of several genes associated with inflammation in both groups of injured animals. Genes connected to the process of angiogenesis, proliferation, differentiation, oxidative stress and extracellular matrix formation were without statistically significant changes. Conclusion: The evidence did not support that HBO2 had any significant effect on gene expression during wound healing. Additionally, there was no evidence to support that there were changes in gene expression in either treatment group.

• MeSH
• chirurgická rána genetika   terapie   MeSH
• čipová analýza tkání metody   MeSH
• exprese genu * MeSH
• hojení ran genetika   MeSH
• hyperbarická oxygenace * MeSH
• králíci MeSH
• kůže zranění   MeSH
• messenger RNA analýza   MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH