A novel tritimovirus of the family Potyviridae was isolated from tall oatgrass, Arrhenatherum elatius, exhibiting mosaic symptoms. The virus, for which the name tall oatgrass mosaic virus (TOgMV) is coined, has a filamentous particle of 720 nm and is associated with pinwheel inclusion bodies characteristic of members of the family Potyviridae. The virus was mechanically transmitted to tall oatgrass seedlings, which subsequently exhibited mosaic symptoms. The experimental host range was limited to a few monocot species. The complete genome sequence of TOgMV was determined to be 9359 nucleotides, excluding the 3' polyadenylated tail. The viral RNA encodes one large putative open reading frame of 3029 amino acids with a genome organization typical of monopartite potyvirids. Pairwise comparison of putative mature proteins and proteinase cleavage sites indicated that TOgMV is most closely related to members of the genus Tritimovirus. Phylogenetic analysis of the complete polyprotein and CP sequences of representative members of the family Potyviridae indicate that TOgMV is a distinct tritimovirus naturally infecting tall oatgrass.
- MeSH
- druhová specificita MeSH
- fylogeneze MeSH
- lipnicovité virologie MeSH
- listy rostlin virologie MeSH
- nemoci rostlin virologie MeSH
- Potyviridae klasifikace genetika izolace a purifikace fyziologie MeSH
- regulace exprese virových genů fyziologie MeSH
- virové proteiny genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
Yellow oat-grass plants (Trisetum flavescens L.) with mild mosaic and pronounced dwarfing symptoms were observed at different locations in the Czech Republic. Electron microscope observations of symptomatic plants revealed the presence of filamentous particles and inclusion bodies characteristic of the family Potyviridae. The virus was readily mechanically transmitted to its original host plus a narrow host range of monocot species. Serological assays of infected plant extracts using antiserum specific to the closest species in the family Potyviridae were negative. The 3' end of the viral genome was cloned, sequenced and compared to sequences of species in the family Potyviridae. The virus is more closely related to viruses in the genus Tritimovirus than to other genera within the Potyviridae. Based on phylogenetic analyses of the coat protein cistron and flanking genomic regions, we propose this is a distinct viral species of the genus Tritimovirus, tentatively named Yellow oat-grass mosaic virus (YOgMV).
- MeSH
- buněčná inkluze virologie MeSH
- fylogeneze MeSH
- lipnicovité virologie MeSH
- molekulární sekvence - údaje MeSH
- nemoci rostlin virologie MeSH
- Potyviridae klasifikace genetika izolace a purifikace MeSH
- RNA virová genetika MeSH
- sekvence aminokyselin MeSH
- sekvenční analýza DNA MeSH
- sekvenční homologie MeSH
- sekvenční seřazení MeSH
- shluková analýza MeSH
- virion ultrastruktura MeSH
- virové plášťové proteiny genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
