Primary cilia play critical roles in development and disease. Their assembly and disassembly are tightly coupled to cell cycle progression. Here, we present data identifying KIF14 as a regulator of cilia formation and Hedgehog (HH) signaling. We show that RNAi depletion of KIF14 specifically leads to defects in ciliogenesis and basal body (BB) biogenesis, as its absence hampers the efficiency of primary cilium formation and the dynamics of primary cilium elongation, and disrupts the localization of the distal appendage proteins SCLT1 and FBF1 and components of the IFT-B complex. We identify deregulated Aurora A activity as a mechanism contributing to the primary cilium and BB formation defects seen after KIF14 depletion. In addition, we show that primary cilia in KIF14-depleted cells are defective in response to HH pathway activation, independently of the effects of Aurora A. In sum, our data point to KIF14 as a critical node connecting cell cycle machinery, effective ciliogenesis, and HH signaling.
- MeSH
- adaptorové proteiny signální transdukční metabolismus MeSH
- aurora kinasa A antagonisté a inhibitory genetika metabolismus MeSH
- bazální tělíska metabolismus MeSH
- buněčný cyklus genetika MeSH
- chromatografie kapalinová MeSH
- cilie genetika metabolismus patologie MeSH
- HEK293 buňky MeSH
- interfáze fyziologie MeSH
- intracelulární signální peptidy a proteiny genetika metabolismus MeSH
- kineziny genetika metabolismus MeSH
- lidé MeSH
- mitóza genetika MeSH
- onkogenní proteiny genetika metabolismus MeSH
- protein-serin-threoninkinasy genetika metabolismus MeSH
- proteiny hedgehog metabolismus MeSH
- RNA interference MeSH
- signální transdukce genetika MeSH
- sodíkové kanály metabolismus MeSH
- tandemová hmotnostní spektrometrie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
